Now showing items 1-5 of 5

    • Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae 

      Liu, Zhuo; Inokuma, Kentaro; Ho, Shih‑Hsin; Den Haan, Riaan; Hasunuma, Tomohisa; Van Zyl, Willem H.; Kondo, Akihiko (BioMed Central, 2015-09-26)
      Background: Engineering Saccharomyces cerevisiae to produce heterologous cellulases is considered as a promising strategy for production of bioethanol from lignocellulose. The production of cellulase is usually pursued ...

    • Engineering cellulolytic ability into bioprocessing organisms 

      La Grange, Daniel C.; Den Haan, Riaan; Van Zyl, Willem H. (Springer-Verlag, 2010-03)
      Lignocellulosic biomass is an abundant renewable feedstock for sustainable production of commodities such as biofuels. The main technological barrier that prevents widespread utilization of this resource for production of ...

    • Engineering of Pichia stipitis for enhanced xylan utilization 

      Den Haan, Riaan (Stellenbosch : Stellenbosch University, 2003-12)
      ENGLISH ABSTRACT: Plant biomass, the most abundant renewable resource in nature, consists of matrices of mainly lignin, cellulose, hemicellulose as well as inorganic components. Xylan, the major hemicellulose component ...

    • Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol 

      Den Haan, Riaan; Mcbride, John E.; La Grange, Daniel C.; Lynd, Lee R.; Van Zyl, Willem H. (Elsevier, 2006-09)
      To investigate the possible use of Saccharomyces cerevisiae as a candidate for consolidated bioprocessing (CBP) of cellulose to ethanol, four fungal cellobiohydrolase (CBH) encoding genes (Trichoderma reesei cbh1 and cbh2, ...

    • High level secretion of cellobiohydrolases by Saccharomyces cerevisiae 

      Ilmen, Marja; Den Haan, Riaan; Brevnova, Elena; McBride, John; Wiswall, Erin; Froehlich, Allan; Koivula, Anu; Voutilainen, Sanni P.; Siika-aho, Matti; LaGrange, Daniel C.; Thorngren, Naomi; Ahlgren, Simon; Mellon, Mark; Deleault, Kristen; Rajgarhia, Vineet; Van Zyl, Willem H.; Penttila, Merja (BioMed Central, 2011-09)
      BACKGROUND: The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance. Organisms that hydrolyze ...