Subject
Now showing items 1-20 of 74
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Analysis of an 18kb accessory region of plasmid pTcM1 from Acidithiobacillus caldus MNG
(Stellenbosch : University of Stellenbosch, 2009-03)Biomining organisms are generally found in metal-rich, inorganic environments such as iron and sulfur containing ores; where they play a vital role in mineralization and decomposition of minerals. They are typically ... -
Analysis of arsenic resistance in the biomining bacterium, Acidithiobacillus caldus
(Stellenbosch : University of Stellenbosch, 2007-03)ENGLISH ABSTRACT: In this study the chromosomal arsenic resistance (ars) genes shown to be present in all Acidithiobacillus. caldus isolates were cloned and sequenced from At. caldus #6. Ten open reading frames (ORFs) were ... -
Assessing the occurrence and mechanisms of horizontal gene transfer during wine making
(2009-12)ENGLISH ABSTRACT: Saccharomyces cerevisiae is the most commonly used organism in many fermentation-based industries including baking and the production of single cell proteins, biofuel and alcoholic beverages. In the ... -
Assessment of wood degradation by Pycnoporus sanguineus when co-cultured with selected fungi
(Stellenbosch : Stellenbosch University, 2007-03)ENGLISH ABSTRACT: It is commonly known that a diversity of fungi, including yeasts, may occur on plant surfaces. Similarly, on fallen trees an ecological succession of different fungal species is known to occur during ... -
Biodegradation of winery wastewater
(Stellenbosch : University of Stellenbosch, 2003-04)ENGLISH ABSTRACT: Large volumes of wastewater are generated annually during the grape harvest season from various processing and cleaning operations at wineries, distilleries and other wine-related industries. South ... -
Biodiversity in the genus Penicillium from coastal fynbos soil
(Stellenbosch : Stellenbosch University, 2008-12)Penicillium is a well‐known cosmopolitan genus with more than 225 accepted species. Species from this diverse genus, in general, are considered to primarily be soil fungi, with decomposition as its main function. Therefore, ... -
The biology, diversity and evolution of the broad host-range, promiscuous INCQ plasmids, with an emphasis on the INCQ2 sub-family
(Stellenbosch : Stellenbosch University, 2014-12)ENGLISH ABSTRACT: Plasmids belonging to the IncQ family have an exceptionally broad host-range and are highly mobilizable in the presence of the self-transmissible IncP plasmids. All IncQ plasmids identified to date have ... -
Bioprospecting for beta-glucosidases and beta-xylosidases from non-Saccharomyces yeast
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: The argument of whether to use food for biofuel (bioethanol) production prompted the search for an alternative non-food biomass, such as lignocellulose, as feedstock for bioethanol production. However, ... -
Characterisation of plasmid p31T1 isolated from Aeromonas
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: Plasmids are an integral part of the horizontal gene pool and, therefore, are the main vectors for the spread of antibiotic and heavy metal resistance genes in the environment. Functional and taxonomic ... -
Characterisation of the malate transporter and malic enzyme from Candida utilis
(Stellenbosch : University of Stellenbosch, 2011-10)ENGLISH ABSTRACT: Yeast species differ remarkably in their ability to degrade extracellular dicarboxylic acids and to utilise them as their only source of carbon. The fission yeast Schizosaccharomyces pombe effectively ... -
Characterization of a broad-spectrum antimicrobial peptide from Enterococcus mundtii active against bacteria associated with middle ear infections
(Stellenbosch : University of Stellenbosch, 2006-12)ENGLISH ABSTRACT: Strain ST4SA, isolated from soya beans, was identified as Enterococcus mundtii. BacST4SA, a bacteriocin produced by strain ST4SA inhibited the growth of Acinetobacter baumannii, Bacillus cereus, Clostridium ... -
Characterization of bacteriocins produced by lactic acid bacteria from fermented beverages and optimization of starter cultures
(Stellenbosch : Stellenbosch University, 2008-03)Lactobacillus plantarum JW3BZ and Lactobacillus fermentum JW15BZ isolated from boza, a Bulgarian cereal based fermented beverage, produce bacteriocins JW3BZ and JW15BZ active against a wide range of food spoilage and ... -
Characterization of nisin F and its role in the control of respiratory tract and skin infections
(Stellenbosch : University of Stellenbosch, 2009-03)Multidrug resistant strains of Staphylococcus aureus is presenting an increasing threat, especially immune compromised individuals. Many of these strains have developed resistance to newly approved drugs such as ... -
Characterization of the adhesion genes of probiotic lactic acid bacteria
(Stellenbosch : Stellenbosch University, 2008-03)One of the key selection criteria for potential probiotics is the ability to adhere and colonise the host gastrointestinal tract (GIT). Probiotics compete for receptor sites at the host intestinal surface, preventing ... -
Cloning and expression of a β-glucosidase gene from Acremonium cellulolyticus in Saccharomyces cerevisiae
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Humanity is currently dependant on fossil fuels as an energy source. Increasing economic development and industrialization is, however, raising the demand for this unsustainable energy source. This ... -
Cloning and functional expression of three xylanase genes from Aspergillus fumigatus in Saccharomyces cerevisiae
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Lignocellulose, which is composed of cellulose, hemicellulose and lignin, is the main structural component of plant cell walls. Xylan is the main structural component of hemicellulose. Xylan is a complex ... -
Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f.
(Stellenbosch : University of Stellenbosch, 2005-03)Plasmid pTC-F14 is a 14.2kb promiscuous, broad-host range IncQ-like mobilizable plasmid isolated from Acidithiobacillus caldus f. At. caldus is a member of a consortium of bacteria (along with Acidithiobacillus ferrooxidans ... -
Co-expression of cellulase genes in Saccharomyces cerevisiae for cellulose degradation
(Stellenbosch : Stellenbosch University, 2008-12)Complete degradation of cellulose produces mainly glucose, which can be fermented to ethanol. Therefore cellulose presents an abundant renewable energy resource for the production of an alternative, environmentally ... -
Community-level analysis of the microbiology in constructed wetlands treating distillery effluent
(Stellenbosch : University of Stellenbosch, 2006-04)ENGLISH ABSTRACT: Constructed wetlands have been widely used in the treatment of industrial and domestic wastewater to reduce biological and chemical oxygen demand (BOD and COD), to remove nitrate and enteric viruses as ... -
Comparative proteomic and genomic analysis of Flavobacterium johnsoniae-like biofilm, planktonic and agar surface-associated cells
(Stellenbosch : University of Stellenbosch, 2010-03)ENGLISH ABSTRACT: Pathogenic Flavobacterium spp. cause serious disease outbreaks in a variety of farmed fish, which lead to large economic losses in the aquaculture industry on an annual basis. The ability of Flavobacterium ...
