Browsing Department of Microbiology by Title
Now showing items 68-87 of 318
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Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f.
(Stellenbosch : University of Stellenbosch, 2005-03)Plasmid pTC-F14 is a 14.2kb promiscuous, broad-host range IncQ-like mobilizable plasmid isolated from Acidithiobacillus caldus f. At. caldus is a member of a consortium of bacteria (along with Acidithiobacillus ferrooxidans ... -
Co-detection of virulent escherichia coli genes in surface water sources
(Public Library of Science, 2015)McNemar’s test and the Pearson Chi-square were used to assess the co-detection and observed frequency, respectively, for potentially virulent E. coli genes in river water. Conventional multiplex Polymerase Chain Reaction ... -
Co-expression of cellulase genes in Saccharomyces cerevisiae for cellulose degradation
(Stellenbosch : Stellenbosch University, 2008-12)Complete degradation of cellulose produces mainly glucose, which can be fermented to ethanol. Therefore cellulose presents an abundant renewable energy resource for the production of an alternative, environmentally ... -
Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae
(Springer-Verlag, 1999-12)The xynB gene encoding the Bacillus pumilus beta-xylosidase was expressed separately and jointly with the Trichoderma reesei beta-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the ... -
Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae
(BioMed Central, 2015-09-26)Background: Engineering Saccharomyces cerevisiae to produce heterologous cellulases is considered as a promising strategy for production of bioethanol from lignocellulose. The production of cellulase is usually pursued ... -
Community-level analysis of the microbiology in constructed wetlands treating distillery effluent
(Stellenbosch : University of Stellenbosch, 2006-04)ENGLISH ABSTRACT: Constructed wetlands have been widely used in the treatment of industrial and domestic wastewater to reduce biological and chemical oxygen demand (BOD and COD), to remove nitrate and enteric viruses as ... -
Comparative analysis of solar pasteurization versus solar disinfection for the treatment of harvested rainwater
(BioMed Central, 2016-12-09)Background: Numerous pathogens and opportunistic pathogens have been detected in harvested rainwater. Developing countries, in particular, require time- and cost-effective treatment strategies to improve the quality ... -
Comparative proteomic and genomic analysis of Flavobacterium johnsoniae-like biofilm, planktonic and agar surface-associated cells
(Stellenbosch : University of Stellenbosch, 2010-03)ENGLISH ABSTRACT: Pathogenic Flavobacterium spp. cause serious disease outbreaks in a variety of farmed fish, which lead to large economic losses in the aquaculture industry on an annual basis. The ability of Flavobacterium ... -
Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse
(BioMed Central, 2013-11)Background: The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for ... -
Comparative study for the transformation of emerging contaminants and endocrine disrupting compounds : electrochemical oxidation and biological metabolism
(Stellenbosch : Stellenbosch University, 2020-03)ENGLISH ABSTRACT: As little as 1% of all water sources is fresh water accessible for use and it is increasingly be polluted by anthropogenic materials such as solid and chemical waste. Previous studies have shown that ... -
Comparison of quantitative techniques including Xpert MTB/RIF to evaluate mycobacterial burden
(PLOS, 2011-12)Introduction: Accurate quantification of mycobacterial load is important for the evaluation of patient infectiousness, disease severity and monitoring treatment response in human and in-vitro laboratory models of disease. ... -
Construction of cellobiose-growing and fermenting Saccharomyces cerevisiae strains
(Elsevier, 2004-12)β-Glucosidase genes of fungal origins were isolated and heterologously expressed in Saccharomyces cerevisiae to enable growth on the disaccharide, cellobiose. To promote secretion of the β-glucosidases, the genes were fused ... -
Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch
(BMC (part of Springer Nature), 2019-08-20)Background: Consolidated bioprocessing (CBP) combines enzyme production, saccharification and fermentation into a one-step process. This strategy represents a promising alternative for economic ethanol production from starchy ... -
Control of bacterial pathogens associated with mastitis in dairy cows with natural antimicrobial peptides produced by lactic acid bacteria
(Stellenbosch : Stellenbosch University, 2008-03)Mastitis is considered to be the most costly disease affecting the dairy industry. Management strategies involve the extensive use of antibiotics to treat and prevent this disease. Prophylactic dosages of antibiotics ... -
Control of malolactic fermentation in wine. a review
(South African Society for Enology and Viticulture, 2004)Malolactic fermentation (MLF) is conducted by lactic acid bacteria (LAB) and refers to the decarboxylation of Lmalate to L-lactate. This secondary fermentation is difficult to control and is mainly driven by Oenococcus ... -
Converting plant biomass to fuels and commodity chemicals in South Africa : a third chapter?
(Academy of Science for South Africa, 2003)THERE HAVE BEEN TWO DISTINCT CHAPTERS in the history of converting cellulosic biomass to fuels and commodity chemicals in South Africa. The first chapter, from the late 1970s to the early 1990s, involved some of the most ... -
Copper-containing anti-biofilm nanofiber scaffolds as a wound dressing material
(Public Library of Science, 2016)Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus ... -
Covalent immobilisation of β-Galactosidase from Escherichia coli to commercially available magnetic nanoparticles for the removal of lactose from milk
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: ß-Galactosidase of Escherichia coli is the equivalent of lactase in humans and has the ability to bind and hydrolyse lactose. Lactase de ciency is a common phenomenon present in almost 70% of the world's ... -
Crossbreeding of natural Saccharomyces cerevisiae strains for enhanced bio-ethanol production
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: The fluctuating fuel prices, possible future shortage of fossil fuels, the increasing demand and the negative impacts on the ecosystem have all contributed to the search and development of alternatives ... -
Cytochrome P450 polymorphisms : relevance in two South African disease populations
(Stellenbosch : Stellenbosch University, 2003-12)ENGLISH ABSTRACT: With knowledge of the human genome increasing constantly we are continually faced with new and potentially groundbreaking methods for managing, treating and/or identifying diseases and predisposition ...
