Browsing Department of Microbiology by Title
Now showing items 169-188 of 317
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Heterologous expression of a fungal lignin peroxidase in Pichia pastoris
(Stellenbosch : Stellenbosch University, 2019-03)ENGLISH ABSTRACT: Considerable research has been being devoted to seek cost-effective and environmentally friendly alternatives to replace fossil fuel-based resources for energy and other essential products. Lignocellulosic ... -
Heterologous expression of cellulase genes in natural Saccharomyces cerevisiae strains
(Stellenbosch : Stellenbosch University, 2016-03)ENGLISH ABSTRACT: The yeast Saccharomyces cerevisiae is regularly chosen for the heterologous production of industrial and medically relevant proteins, due to its rapid growth rate, high cell density fermentation capabilities, ... -
Heterologous expression of the class IIa bacteriocins, plantaricin 423 and mundticin ST4SA, in escherichia coli using green fluorescent protein as a fusion partner
(Frontiers Media, 2020)The antilisterial class IIa bacteriocins, plantaricin 423 and mundticin ST4SA, have previously been purified from the cell-free supernatants of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA, respectively. Here, ... -
Heterologous production of family 5 fungal endo-1,4-B-mannanases in Saccharomyces cerevisiae
(Stellenbosch : Stellenbosch University, 2002-12)ENGLISH ABSTRACT: Mannan polysaccharides occur in the hemicellulose fraction of plant cell walls, either as structural polymers or as reserve carbohydrates. They are found predominantly in the seeds of leguminous plants ... -
High level secretion of cellobiohydrolases by Saccharomyces cerevisiae
(BioMed Central, 2011-09)BACKGROUND: The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance. Organisms that hydrolyze ... -
High yield of culture-based diagnosis in a TB-endemic setting
(BioMed Central, 2012-09)Abstract Background In most of the world, microbiologic diagnosis of tuberculosis (TB) is limited to microscopy. Recent guidelines recommend culture-based diagnosis where feasible. ... -
High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system
(BioMed Central, 2009-10)Abstract Background Some strains of Bacillus licheniformis have been improved by target-directed screening as well as by ... -
Highly efficient L-lactate production using engineered Escherichia coli with dissimilar temperature optima for L-lactate formation and cell growth
(BioMed Central, 2014)L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase. Naturally, Escherichia ... -
How do honey bees handle their stress? A focus on their gut microbiota and immune system. (Apis mellifera subsp. capensis)
(Stellenbosch : Stellenbosch University, 2018-12)ENGLISH ABSTRACT: Gut microbial symbionts have recently been shown to play roles in ensuring overall host health, a hot topic in honey bee research. Honey bees harbour a stable, core bacterial community in the gut, suggested ... -
Identification and characterisation of a Cryptococcus laurentii Abo 510 Phytase
(Stellenbosch : Stellenbosch University, 2004-03)ENGLISH ABSTRACT: Phosphorus is vital for growth of all life forms and is a fundamental component of nucleic acids, ATP and several other biological compounds. Oilseeds and cereal grains, two major constituents of the ... -
The identification and characterisation of the arsenic resistance genes of the gram-positive bacterium, Sulfobacillus thermosulfidooxidans VKM B-1269T
(Stellenbosch : Stellenbosch University, 2007-03)ENGLISH ABSTRACT: The arsenic resistance operon (ars operon) of the Gram-positive, iron-oxidizing, acidophilic, moderately thermophilic bacterium, Sulfobacillus thermosulfidooxidans VKM B-1269T (Sb. t. VKM B-1269T), was ... -
Identification of lactic acid bacteria isolated from South African fortified wines
(Stellenbosch : Stellenbosch University, 1999-12)ENGLISH SUMMARY: During the primary fermentation of wine, grape must is fermented to ethanol, CO2 and flavour compounds by Saccharomyces cerevisiae. During the secondary fermentation malolactic bacteria converts L-malic ... -
Identification of lactic acid bacteria isolated from the urogenital tract of women
(Stellenbosch : Stellenbosch University, 1999-12)ENGLISH SUMMARY: Lactic acid bacteria are present in the oral cavity, gastrointestinal tract, vagina, plants, fermented foods, manure and sewage (Kandler & Weiss, 1986). These organisms are acidophilic and thrive at a ... -
Identification of lactic acid bacteria isolated from vinegar flies and Merlot grapes
(Stellenbosch : University of Stellenbosch, 2011-10)ENGLISH ABSTRACT: Thirty lactic acid bacteria were isolated from the intestinal tract of Drosophila simulans Stuvervant and nine lactic acid bacteria from Merlot grapes collected from the same winery in the Stellenbosch ... -
Identification of lactobacillus spp. isolated from different phases during the production of a South African fortified wine
(South African Society for Enology and Viticulture, 2002)Fortified wines contain a high level of unfermented sugars and are prone to spoilage by alcohol-tolerant lactic acid bacteria. A total of 62 strains were isolated from various production stages of one of the more popular ... -
Identification of the gene for β-fructofuranosidase from Ceratocystis moniliformis CMW 10134 and characterization of the enzyme expressed in Saccharomyces cerevisiae
(BioMed Central, 2013-11)Background: β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in the functional foods ... -
Identifying the primary microbial and chemical source tracking markers in harvested rainwater for the detection of faecal contamination
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: Rainwater harvesting has been earmarked as an additional source of fresh water. However, research has indicated that the microbiological quality is substandard as pathogens have been detected in this water ... -
The impact of copper on filamentous fungi and yeasts present in soil
(Stellenbosch : University of Stellenbosch, 2004-04)ENGLISH ABSTRACT: Numerous workers studied the impact of pollutants and agricultural chemicals, containing heavy metals such as copper (Cu), on soil microbes. It was found that elevated soil Cu levels do have a detrimental ... -
Impact of inhibitors associated with lignocellulosic hydrolysates on recombinant cellulolytic enzymes
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: Enzymatic hydrolysis contributes a significant cost towards the production of bioethanol and is estimated to comprise 15% of the minimum ethanol selling price. One of the areas of concern during the ... -
Impact of yeast breeding for elevated glycerol production on fermentative activity and metabolite formation in chardonnay wine
(South African Society for Enology and Viticulture, 2000)Glycerol in wine originates mainly as a by-product during fermentation by yeast and is thought to add to the body and smooth mouth-feel. We evaluated the properties of Chardonnay wine produced using various wine yeast ...
