Browsing Department of Microbiology by Title
Now showing items 88-107 of 317
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Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger β-xylosidase (xlnD) and the Trichoderma reesei Xylanase II (xyn2) genes
(American Society of Microbiology, 2001-12)The β-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified ... -
Deletion analysis of the Ure2p in Saccharomyces cerevisiae and effect of NCR on the production of ethyl carbamate during wine fermentations
(Stellenbosch : Stellenbosch University, 2000-12)ENGLISH ABSTRACT: The wine yeast Saccharomyces cerevisiae has the ability to utilize several different nitrogenous compounds to fulfill its metabolic requirements. Based upon different growth rates of the yeast in a ... -
Detection of biological false positive syphilis serum reactions
(Health & Medical Publishing Group, 1974)A comparative evaluation of reagin tests (Wassermann, VDRL, RPR) and fluorescent treponema antibody absorption tests (FTA ABS) performed on blood specimens from 5,271 persons (2,493 pregnant women, 1,130 apparently healthy ... -
Detection, identification and quantitation of Cryptosporidium parvum in water samples and Ascaris lumbricoides in sludge samples using real-time Polymerase Chain Reaction coupled with the high-resolution melt curve assay
(Stellenbosch : Stellenbosch University, 2016-03)ENGLISH ABSTRACT: The intestinal parasites Ascaris lumbricoides and Cryptosporidium parvum are highly resistant to traditional water treatment processes and adverse health effects in individuals that come into contact with ... -
Determination of the bacterial diversity of a natural freshwater wetland impacted by acid mine drainage.
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: Freshwater resources in semi-arid countries, such as South Africa, are under constant threat from pollution. One of the major pollutants is acid mine drainage, which not only lowers the pH of the water, ... -
Developing bone cement implants impregnated with bacteriocins for prevention of infections
(Stellenbosch : Stellenbosch University, 2011-12)ENGLISH ABSTRACT: Infection is one of the major causes of increased morbidity and the escalating costs associated with orthopedic surgery. The areas that are infected are often difficult to reach and thus difficult to ... -
Development of a ZnO nanowire-array biosensor for the detection and quantification of immunoglobulins
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: The aim of this study was to develop a ZnO nanowire-array biosensor that would detect immunoglobulins and record changes in the concentration of an antibody. Early detection of disease-causing agents is ... -
Development of an Agrobacterium vitis transformation system for grapevine
(Stellenbosch : Stellenbosch University, 2000-03)ENGLISH ABSTRACT: Agrobacterium tumefaciens-mediated transformation technology has been used in a variety of applications throughout the fields of cellular and molecular plant biology as well as plant physiology. Research ... -
Development of an antimicrobial wound dressing by co-electrospinning bacteriocins of lactic acid bacteria into polymeric nanofibers
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: Skin is the largest organ in the human body and serves as a barrier that protects the underlying tissue of the host from infection. Injury, however, destroys this protective barrier and provides a perfect ... -
Development of improved α-amylases
(Stellenbosch : University of Stellenbosch, 2005-03)The technological advancement of modern human civilisation has, until recently, depended on extensive exploitation of fossil fuels, such as oil, coal and gas, as sources of energy. Over the last few decades, greater ... -
The development of recombinant fungal enzyme cocktails for the hydrolysis of cellulosic waste products
(Stellenbosch : Stellenbosch University, 2014-12)ENGLISH ABSTRACT: Biofuels, such as bioethanol, provide an alternative, environmentally friendly transportation fuel. Renewable energy sources, such as lignocellulosic material, are therefore being explored for the production ... -
Development of recombinant Saccharomyces cerevisiae for improved D-xylose utilisation
(Stellenbosch : University of Stellenbosch, 2006-04)ENGLISH ABSTRACT: Plant biomass is potentially an inexhaustible source of bioenergy. To be more useful in an industrialised context, conversion to liquid biofuel is necessary, which could provide the motor vehicle market ... -
Diagnosis and incidence of Neisseria gonorrhoeae in Cape Coloured females in the Western Cape : laboratory aspects
(Health & Medical Publishing Group, 1974)Specimens were taken, using carbon impregnated swabs, from the cervix, urethra and rectrum of 945 Cape Coloured gynaecological patients, and from the cervix only of 1,276 pregnant Cape Coloured women. These specimens were ... -
Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae alpha-amylase in industrial strains of Saccharomyces cerevisiae
(Kluwer (now part of Springer), 2002-01)A haploid laboratory strain and four industrial (baking, brewing, wine, ATCC) strains of Saccharomyces cerevisiae were transformed with the Lipomyces kononenkoae alpha-amylase-encoding gene (LKA1). These transformants ... -
Differential response of sessile and planktonic bacterial populations following exposure to antimicrobial treatment
(Stellenbosch : Stellenbosch University, 2004-04)ENGLISH ABSTRACT: The ability of biofilms to resist antimicrobial treatment, when planktonic microbes cannot, is of not only fundamental scientific interest, but also a concern in industrial and medical fields. The ... -
Diguanylate cyclase null mutant reveals that C-Di-GMP pathway regulates the motility and adherence of the extremophile bacterium acidithiobacillus caldus
(Public Library of Science, 2015)An understanding of biofilm formation is relevant to the design of biological strategies to improve the efficiency of the bioleaching process and to prevent environmental damages caused by acid mine/rock drainage. For this ... -
Diversity and ontogeny of Cryptococcus neoformans var. grubii originating from South Africa
(Stellenbosch : Stellenbosch University, 2007-12)ENGLISH ABSTRACT: Cryptococcus neoformans (Sanfelice) Vuillemin is an opportunistic fungal pathogen responsible for causing meningitis predominantly in immuno-compromised individuals, particularly in those suffering from ... -
The diversity of Trichoderma spp. in South Africa
(Stellenbosch : Stellenbosch University, 2015-03)ENGLISH SUMMARY: The genus Trichoderma includes economically important species that are routinely applied in fields such as agriculture and biotechnology. Species like T. harzianum and T. asperellum are commercially applied ... -
Ecology of biological sulfate removal
(Stellenbosch : Stellenbosch University, 2003-04)ENGLISH ABSTRACT: A laboratory-scale model was used to simulate biological sulfate removal. The focus of the research was microbial community response, such as the relative abundance of functional groups to changes in ... -
Economic risks due to declining water quality in the Breede River catchment
(South African Water Research Commission, 2018-07-03)Water is a critical resource necessary to support social and economic development. Economic growth and, in particular, the growth of urban and peri-urban areas, however results in declining water quality which threatens ...
