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Applications of generalised supply-demand analysis

Christensen, Carl David (Stellenbosch : Stellenbosch University, 2013-03)

ENGLISH ABSTRACT: Supply-demand analysis (SDA) is a tool that allows for the control, regulation and behaviour of metabolic pathways to be understood. In this framework, reactions are grouped into reaction blocks that ...

An assessment of the mutation patterns in South African isolates of Potato leafroll virus and the expression of recombinant viral coat protein genes in Escherichia coli

Rothmann, Adri Hilda (Stellenbosch : Stellenbosch University, 2007-03)

ENGLISH ABSTRACT: Presently, the observed variation in symptoms of Potato leafroll virus (PLRV) infection in potato cultivars in South Africa cannot be reconciled with PLRV symptoms obtained 10-15 years ago, even if the ...

Bioaffinity separation using ligand-modified pluronic and synthetic membranes

Govender, Selvakumaran (Stellenbosch : University of Stellenbosch, 2011-10)

ENGLISH ABSTRACT: A new membrane based affinity separation system that is bio-specific, biocompatible, well characterised and capable of being regenerated or re-used is described. The amphiphilic non-ionic surfactant ...

A biochemical and immunological study of horseradish peroxidase

Odendaal, Ruenda (Stellenbosch : University of Stellenbosch, 2009-12)

ENGLISH ABSTRACT: This study describes: a) the isolation and purification of horseradish peroxidase isoenzymes from horseradish roots, b) the characterization of various forms and components of the enzyme by cation-exchange ...

A biochemical study of tissue type plasminogen activator in bovine milk

Cilliers, Frans Pieter (Stellenbosch : Stellenbosch University, 2007-03)

ENGLISH ABSTRACT: This study describes: 1. The isolation and the purification of tissue type plasminogen activator and urokinase plasminogen activator in bovine milk. 2. The biochemical characterisation of tissue type ...

Cancer modulating properties of unique South African herbal teas (rooibos and honeybush) in short term in vitro and in vivo carcinogenesis assays

Marnewick, Jeanine Lucasta (Stellenbosch : Stellenbosch University, 2004-12)

ENGLISH ABSTRACT: This thesis provides the first scientific evidence on the cancer modulating properties of two unique South African herbal teas, rooibos (Aspalathus Iinearis) and honeybush (Cyclopia intermedia) utilizing ...

Cape baboon Cytochrome P450 11β-hydroxylases : the characterization of two functional enzymes

Brown, Natasja (Stellenbosch : Stellenbosch University, 2007-03)

ENGLISH ABSTRACT: This study: 1. Describes the localization of CYP11B1 in the Cape baboon adrenal gland using Western blot analysis. CYP11B1 was localized to the adrenal cortex and medulla. 2. Describes the catalytic ...

Characteristics and adaptation of skeletal muscle to endurance exercise

Kohn, Tertius A. (Stellenbosch : University of Stellenbosch, 2011-10)

ENGLISH ABSTRACT: Skeletal muscle adapts to stimuli by modifying structural and metabolic protein expression. Furthermore, a muscle group may vary within itself to accommodate specialisation in regions. Structural and ...

A comparative analysis of the G1/S transition control in kinetic models of the eukaryotic cell cycle

Conradie, Riaan (Stellenbosch : University of Stellenbosch, 2009-12)

ENGLISH ABSTRACT: The multiplication of cells proceeds through consecutive phases of growth and division (G1, S, G2 and M phases), in a process known as the cell cycle. The transition between these phases is regulated ...

Comparative characterization and mutational analysis of type III pantothenate kinases

Brand, Leisl Anne (Stellenbosch : University of Stellenbosch, 2006-03)

This thesis reports the cloning, overexpression and characterization of the coaX gene product from Bacillus subtilis and its homologue from Helicobacter pylori. It demonstrates that these proteins have pantothenate kinase ...

Comparative cross-species analysis of detailed kinetic models of glycolysis

Du Preez, Franco B. (Stellenbosch : University of Stellenbosch, 2009-12)

ENGLISH ABSTRACT: With the recent advances in the field of molecular biology, there is an increased need to integrate data on the various constituents of the cell in kinetic models that can predict and describe cellular ...

Comparative study of the molecular mechanism of action of the synthetic progestins, Medroxyprogesterone acetate and Norethisterone acetate

Africander, Donita Jean (Stellenbosch : University of Stellenbosch, 2010-03)

ENGLISH ABSTRACT: Medroxyprogesterone acetate (MPA) and norethisterone (NET) and its derivatives (norethisterone enanthate (NET-EN); norethisterone acetate (NETA)), are used by millions of women as contraceptives and in ...

Comparison of two CYP17 isoforms : implications for cortisol production in the South African Merino

Hough, Denise (Stellenbosch : Stellenbosch University, 2012-03)

ENGLISH ABSTRACT: This study describes: • the comparison of the enzymatic activities of the two ovine cytochrome P450 17 - hydroxylase/17,20-lyase (CYP17) isoforms expressed in non-steroidogenic COS-1 cells. The Km and ...

Computational systems biology of sucrose accumulation in sugarcane

Uys, Lafras (Stellenbosch : University of Stellenbosch, 2006-03)

This thesis is about mathematical modelling of sucrose accumulation in the storage perenchyma of Saccharum officinarum (sugarcane) In 2001, Rohwer & Botha (76) published a kinetic model that described the defining feature ...

Construction and validation of a detailed kinetic model of glycolysis in asexual Plasmodium falciparum : a feasibility study

Penkler, Gerald Patrick (Stellenbosch : University of Stellenbosch, 2009-12)

ENGLISH ABSTRACT: In Africa alone, Plasmodium, the causative agent of malaria is estimated to kill a child, under the age of five every thirty seconds140. The ability of the parasite to rapidly attain resistance, has ...

Control analysis of adrenal Sseroidogenesis

Genade, Tyrone (Stellenbosch : University of Stellenbosch, 2004-12)

This study describes: 1. Investigation of product inhibition regarding the metabolism of progesterone in ovine adrenal micosomes. 2. The employment of novel cell culture techniques to study the effect of CYP17 and ...

Control analysis of mixed populations of gluconobacter oxydans and saccharomyces cerevisiae

Malherbe, Christiaan Johannes (Stellenbosch : University of Stellenbosch, 2010-12)

ENGLISH ABSTRACT: In the last decade a need arose to find a theoretical framework capable of gaining a quantitative understanding of ecosystems. Control analysis was proposed as a suitable candidate for the analysis of ...

Control analysis of the action potential and its propagation in the Hodgkin-Huxley model

Du Toit, Francois (Stellenbosch : University of Stellenbosch, 2010-12)

ENGLISH ABSTRACT: The Hodgkin-Huxley model, created in 1952, was one of the first models in computational neuroscience and remains the best studied neuronal model to date. Although many other models have a more detailed ...

Coupling kinetic models and advection-diffusion equations to model vascular transport in plants, applied to sucrose accumulation in sugarcane

Uys, Lafras (Stellenbosch : University of Stellenbosch, 2009-12)

ENGLISH ABSTRACT: The sugarcane stalk, besides being the main structural component of the plant, is also the major storage organ for carbohydrates. Sucrose forms the bulk of stored carbohydrates. Previous studies have ...

Development of a synthetic affinity membrane for the purification of recombinant maltose binding proteins

Asongwe, Lionel Ateh Tantoh (Stellenbosch : Stellenbosch University, 2008-12)

The aim of this project was to fabricate a new affinity membrane-based system that is biospecific and biocompatible, and which could be used as an adsorption matrix for the immobilization of the recombinant protein maltose ...

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