The isolation and charcterisation of ovine liver cytochrome b₅

dc.contributor.advisorSwart, P.en_ZA
dc.contributor.advisorSwart, A. C.en_ZA
dc.contributor.authorLombard, Nicolaasen_ZA
dc.contributor.otherStellenbosch University. Faculty of Science. Dept. of Biochemistry.en
dc.descriptionDissertation (PhD)--University of Stellenbosch, 2007.en_ZA
dc.description.abstractENGLISH ABSTRACT: This dissertation describes how the isolation and characterisation of ovine liver cytochrome b5 was accomplished by referring to the following goals achieved in this study: - The optimisation of the isolation and purification procedure for ovine liver microsomal cytochrome b5 in order to obtain sufficient material for aggregation and immunological studies. - The removal of the membrane binding domain of cytochrome b5 by means of tryptic digestion to establish the role of the carboxyl terminal in ovine cytochrome b5 aggregation. - The raising of antibodies against both the trypsin truncated and intact forms of cytochrome b5 to study the aggregation of the protein. - The investigation into the influence of purified cytochrome b5 on steroidogenesis in ovine adrenal microsomes.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: Die isolering en karakterisering van skaaplewersitochroom b5, soos beskryf in hierdie proefskrif, is uitgevoer deur die volgende doelwitte suksesvol af te handel: - Die optimalisering van die prosedure vir die suksesvolle isolering en suiwering van skaaplewersitochroom b5 ten einde genoegsame hoeveelhede van die suiwer proteïen te hê vir die bestudering van die aggregasie van die proteïen sowel as ‘n immunologiese studie. - Die verwydering van die membraanbindingsdomein van sitochroom b5 om die invloed van die karboksielterminaal op die aggregering van die proteïen te bestudeer. - Die gebruik van sowel die tripties gesnyde as die intakte vorms van sitochroom b5 om ‘n immuunrespons in hase op te wek vir die verkryging van sitochroom b5 spesifieke anti-liggame. - Die gebruik van die gesuiwerde proteïene om die invloed van sitochroom b5 op adrenale steroïdogenese te
dc.format.extent153 leaves : ill. (some col.)
dc.publisherStellenbosch : Stellenbosch University
dc.subjectCytochrome ben_ZA
dc.subjectLiver -- Cytochemistryen_ZA
dc.subjectTheses -- Biochemistryen_ZA
dc.subjectDissertations -- Biochemistryen_ZA
dc.titleThe isolation and charcterisation of ovine liver cytochrome b₅en_ZA
dc.rights.holderStellenbosch Universityen_ZA

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