The isolation and charcterisation of ovine liver cytochrome b₅

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dc.contributor.advisor Swart, P.
dc.contributor.advisor Swart, A.C.
dc.contributor.author Lombard, Nicolaas
dc.contributor.other Stellenbosch University. Faculty of Science. Dept. of Biochemistry. en
dc.date.accessioned 2012-02-01T10:49:11Z
dc.date.available 2012-02-01T10:49:11Z
dc.date.issued 2007-03
dc.identifier.uri http://hdl.handle.net/10019.1/19448
dc.description Dissertation (PhD)--University of Stellenbosch, 2007. en_ZA
dc.description.abstract ENGLISH ABSTRACT: This dissertation describes how the isolation and characterisation of ovine liver cytochrome b5 was accomplished by referring to the following goals achieved in this study: - The optimisation of the isolation and purification procedure for ovine liver microsomal cytochrome b5 in order to obtain sufficient material for aggregation and immunological studies. - The removal of the membrane binding domain of cytochrome b5 by means of tryptic digestion to establish the role of the carboxyl terminal in ovine cytochrome b5 aggregation. - The raising of antibodies against both the trypsin truncated and intact forms of cytochrome b5 to study the aggregation of the protein. - The investigation into the influence of purified cytochrome b5 on steroidogenesis in ovine adrenal microsomes. en_ZA
dc.description.abstract AFRIKAANSE OPSOMMING: Die isolering en karakterisering van skaaplewersitochroom b5, soos beskryf in hierdie proefskrif, is uitgevoer deur die volgende doelwitte suksesvol af te handel: - Die optimalisering van die prosedure vir die suksesvolle isolering en suiwering van skaaplewersitochroom b5 ten einde genoegsame hoeveelhede van die suiwer proteïen te hê vir die bestudering van die aggregasie van die proteïen sowel as ‘n immunologiese studie. - Die verwydering van die membraanbindingsdomein van sitochroom b5 om die invloed van die karboksielterminaal op die aggregering van die proteïen te bestudeer. - Die gebruik van sowel die tripties gesnyde as die intakte vorms van sitochroom b5 om ‘n immuunrespons in hase op te wek vir die verkryging van sitochroom b5 spesifieke anti-liggame. - Die gebruik van die gesuiwerde proteïene om die invloed van sitochroom b5 op adrenale steroïdogenese te bestudeer. af
dc.format.extent 153 leaves : ill. (some col.)
dc.language.iso en_ZA en_ZA
dc.publisher Stellenbosch : Stellenbosch University
dc.subject Cytochrome b en_ZA
dc.subject Liver -- Cytochemistry en_ZA
dc.subject Theses -- Biochemistry en_ZA
dc.subject Dissertations -- Biochemistry en_ZA
dc.title The isolation and charcterisation of ovine liver cytochrome b₅ en_ZA
dc.type Thesis en_ZA
dc.rights.holder Stellenbosch University en_ZA


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