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Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

dc.contributor.authorAlexandersson, Erik
dc.contributor.authorBecker, John V. W.
dc.contributor.authorJacobson, Dan
dc.contributor.authorNguema-Ona, Eric
dc.contributor.authorSteyn, Cobus
dc.contributor.authorDenby, Katherine J.
dc.contributor.authorVivier, Melane A.
dc.date.accessioned2011-12-15T08:12:07Z
dc.date.available2011-12-15T08:12:07Z
dc.date.issued2011-11
dc.identifier.citationAlexandersson, E. et al 2011. Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco. BMC Research Notes. 4(1):493-509en_ZA
dc.identifier.urihttp://dx.doi.org/10.1186/1756-0500-4-493
dc.identifier.urihttp://hdl.handle.net/10019.1/18202
dc.descriptionThe original publication is available at http://www.biomedcentral.com/1756-0500/4/493
dc.description.abstractAbstract: Background Polygalacturonase-inhibiting proteins (PGIPs) directly limit the effective ingress of fungal pathogens by inhibiting cell wall-degrading endopolygalacturonases (ePGs). Transgenic tobacco plants over-expressing grapevine (Vitis vinifera) Vvpgip1 have previously been shown to be resistant to Botrytis infection. In this study we characterized two of these PGIP over-expressing lines with known resistance phenotypes by gene expression and hormone profiling in the absence of pathogen infection. Results Global gene expression was performed by a cross-species microarray approach using a potato cDNA microarray. The degree of potential cross-hybridization between probes was modeled by a novel computational workflow designed in-house. Probe annotations were updated by predicting probe-to-transcript hybridizations and combining information derived from other plant species. Comparing uninfected Vvpgip1-overexpressing lines to wild-type (WT), 318 probes showed significant change in expression. Functional groups of genes involved in metabolism and associated to the cell wall were identified and consequent cell wall analysis revealed increased lignin-levels in the transgenic lines, but no major differences in cell wall-derived polysaccharides. GO enrichment analysis also identified genes responsive to auxin, which was supported by elevated indole-acetic acid (IAA) levels in the transgenic lines. Finally, a down-regulation of xyloglucan endotransglycosylase/hydrolases (XTHs), which are important in cell wall remodeling, was linked to a decrease in total XTH activity. Conclusions This evaluation of PGIP over-expressing plants performed under pathogen-free conditions to exclude the classical PGIP-ePG inhibition interaction indicates additional roles for PGIPs beyond the inhibition of ePGs.en_ZA
dc.format.extent17 p. : col. ill.
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.subjectPhytopathogenic microorganisms -- Biological controlen_ZA
dc.subjectTobacco -- Disease and pest resistanceen_ZA
dc.subjectPolygalacturonaseen_ZA
dc.subjectPlant-pathogen relationsen_ZA
dc.titleConstitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobaccoen_ZA
dc.typeArticleen_ZA
dc.date.updated2011-12-14T12:07:36Z
dc.description.versionPublishers' Versionen_ZA
dc.description.versionUnder the Creative Commons Attribution licenseen_ZA
dc.language.rfc3066en
dc.rights.holderAlexandersson et al.; licensee BioMed Central Ltd.en_ZA


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