Proteomic analysis of mitochondrial proteins in a mouse model of type 2 diabetes
Date
2011
Authors
Essop M.F.
Chan W.A.
Hattingh S.
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Abstract
Objective: Impaired mitochondrial function may contribute to the onset of contractile dysfunction with insulin resistance/ type 2 diabetes. Our aim was therefore to determine alterations in the mitochondrial proteome of a mouse model of obesity/type 2 diabetes. Methods: Mitochondrial proteins were isolated from hearts collected from 18- to 20-week-old female db/db mice and compared to matched controls. We performed two-dimensional polyacrylamide gel electrophoresis to determine differentially expressed proteins. Peptides of interest were further analysed by mass spectrometry and Mascot software was employed to identify protein matches. Results: Our data showed that ATP synthase D chain, ubiquinol cytochrome-C reductase core protein 1 and electron transfer flavoprotein subunit alpha peptide levels were altered with obesity. Moreover, we found coordinate downregulation of contractile proteins in the obese heart, i.e. α-smooth muscle actin, α-cardiac actin, myosin heavy-chain α and myosin-binding protein C. Conclusion: We propose that decreased contractile protein levels may contribute to contractile dysfunction of hearts from diabetic mice.
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Cardiovascular Journal of Africa
22
4
http://www.scopus.com/inward/record.url?eid=2-s2.0-80052223023&partnerID=40&md5=3a0f99a2f20486a8adbdadcf50f0b322
22
4
http://www.scopus.com/inward/record.url?eid=2-s2.0-80052223023&partnerID=40&md5=3a0f99a2f20486a8adbdadcf50f0b322