Expression of the malolactic enzyme gene (mle) from Lactobacillus plantarum under winemaking conditions
Date
2011
Authors
Miller B.J.
Franz C.M.A.P.
Cho G.-S.
Du Toit M.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Malolactic fermentation (MLF) plays an important role in the production of wine, especially red wines, resulting in microbial stability, deacidification, as well as contributing to the aroma profile. MLF can be influenced by a number of factors. In this study, the influence of pH and ethanol on expression of the structural malolactic enzyme gene (mle) from Lactobacillus plantarum was investigated in a synthetic wine media, as well as in wine using quantitative PCR. Expression of mle was shown to be inducible by the presence of malic acid, with increased expression in the middle of MLF. Expression of mle was also shown to be increased at low pH values and decreased in the presence of ethanol. This indicates the role of MLF in acid tolerance and the negative impact of ethanol on the completion of MLF. The results therefore provide further evidence that L. plantarum should be applied as co-inoculation for MLF where alcohol will initially not have a negative impact on the malic acid degradation. © 2011 Springer Science+Business Media, LLC.
Description
Keywords
alcohol, bacterial enzyme, malic acid, malolactic enzyme, unclassified drug, acid tolerance, article, bacterial gene, concentration response, controlled study, fermentation, gene expression, gene structure, inoculation, Lactobacillus plantarum, mle gene, nonhuman, nucleotide sequence, pH, polymerase chain reaction, priority journal, wine, Bacterial Proteins, Ethanol, Fermentation, Gene Expression Regulation, Enzymologic, Lactobacillus plantarum, Malate Dehydrogenase, Malates, Wine, Lactobacillus plantarum
Citation
Current Microbiology
62
6
http://www.scopus.com/inward/record.url?eid=2-s2.0-79958246418&partnerID=40&md5=86caa18b12908a378b07e5d41760e0ca
62
6
http://www.scopus.com/inward/record.url?eid=2-s2.0-79958246418&partnerID=40&md5=86caa18b12908a378b07e5d41760e0ca