A rapid PCR-based approach for molecular identification of filamentous fungi

Chen Y. ; Prior B.A. ; Shi G. ; Wang Z. (2011)

Article

In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates © 2011 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.

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