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High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

dc.contributor.authorIlmen, Marja
dc.contributor.authorDen Haan, Riaan
dc.contributor.authorBrevnova, Elena
dc.contributor.authorMcBride, John
dc.contributor.authorWiswall, Erin
dc.contributor.authorFroehlich, Allan
dc.contributor.authorKoivula, Anu
dc.contributor.authorVoutilainen, Sanni P.
dc.contributor.authorSiika-aho, Matti
dc.contributor.authorLaGrange, Daniel C.
dc.contributor.authorThorngren, Naomi
dc.contributor.authorAhlgren, Simon
dc.contributor.authorMellon, Mark
dc.contributor.authorDeleault, Kristen
dc.contributor.authorRajgarhia, Vineet
dc.contributor.authorVan Zyl, Willem H.
dc.contributor.authorPenttila, Merja
dc.date.accessioned2011-10-11T16:08:44Z
dc.date.available2011-10-11T16:08:44Z
dc.date.issued2011-09
dc.identifier.citationIlmén, Marja ... [et al.]. 2011.High level secretion of cellobiohydrolases by Saccharomyces cerevisiae. Biotechnology for biofuels. 4:30, 3-15, http://dx.doi.org/10.1186/1754-6834-4-30.en_ZA
dc.identifier.issn1754-6834
dc.identifier.otherhttp://dx.doi.org/10.1186/1754-6834-4-30
dc.identifier.urihttp://hdl.handle.net/10019.1/16612
dc.descriptionThe original publication is available at http://www.biotechnologyforbiofuels.com/content/4/1/30
dc.description.abstractBACKGROUND: The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance. Organisms that hydrolyze lignocellulose and produce a valuable product such as ethanol at a high rate and titer could significantly reduce the costs of biomass conversion technologies, and will allow separate conversion steps to be combined in a consolidated bioprocess (CBP). Development of Saccharomyces cerevisiae for CBP requires the high level secretion of cellulases, particularly cellobiohydrolases. RESULTS: We expressed various cellobiohydrolases to identify enzymes that were efficiently secreted by S. cerevisiae. For enhanced cellulose hydrolysis, we engineered bimodular derivatives of a well secreted enzyme that naturally lacks the carbohydrate-binding module, and constructed strains expressing combinations of cbh1 and cbh2 genes. Though there was significant variability in the enzyme levels produced, up to approximately 0.3 g/L CBH1 and approximately 1 g/L CBH2 could be produced in high cell density fermentations. Furthermore, we could show activation of the unfolded protein response as a result of cellobiohydrolase production. Finally, we report fermentation of microcrystalline cellulose (Avicel™) to ethanol by CBH-producing S. cerevisiae strains with the addition of beta-glucosidase. CONCLUSIONS: Gene or protein specific features and compatibility with the host are important for efficient cellobiohydrolase secretion in yeast. The present work demonstrated that production of both CBH1 and CBH2 could be improved to levels where the barrier to CBH sufficiency in the hydrolysis of cellulose was overcome.en_ZA
dc.format.extent15 p. : ill.
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.subjectLignocelluloseen_ZA
dc.subjectFuelen_ZA
dc.subjectBiomass conversion technologiesen_ZA
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.subjectCellobiohydrolasesen_ZA
dc.titleHigh level secretion of cellobiohydrolases by Saccharomyces cerevisiaeen_ZA
dc.typeArticleen_ZA
dc.date.updated2011-10-07T05:10:32Z
dc.description.versionPublishers' versionen_ZA
dc.language.rfc3066en
dc.rights.holderIlmen et al.; licensee BioMed Central Ltd.en_ZA


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