Efficiency of co-expression of transcription factors Pdx1, Ngn3, NeuroD and Pax6 with insulin : a statistical approach

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dc.contributor.author Du Toit, Don F.
dc.contributor.author Longo-Mbenza, Benjamin
dc.contributor.author Page, Benedict J.
dc.contributor.author Tchokonte-Nana, Venant
dc.date.accessioned 2011-09-08T13:59:52Z
dc.date.issued 2013-07
dc.identifier.citation Du Toit, D. F., Longo-Mbenza, B., Page, B. J., & Tochokonte-Nana, V. 2013,. Efficiency of co-expression of transcription factors Pdx1, Ngn3, NeuroD and Pax6 with insulin: A statistical approach. International Journal of Statistics in Medical Research, 2(3):229-238, doi:10.6000/1929-6029.2013.02.03.8. en_ZA
dc.identifier.citation Du Toit, D. F., Longo-Mbenza, B., Page, B. J., & Tochokonte-Nana, V. 2011, Efficiency of co-expression of transcription factors Pdx1, Ngn3, NeuroD and Pax6 with insulin: A statistical approach, International Journal of Diabetes Mellitus, http://www.journals.elsevierhealth.com/periodicals/ijdm/inpress, doi:10.1016/j.ijdm 2011.01.007 en_ZA
dc.identifier.uri http://hdl.handle.net/10019.1/16369
dc.description The original publication is available at http://www.lifescienceglobal.com/home/cart?view=product&id=696 en_ZA
dc.description Inpress since 2011 by Elsevier,for the journal, International Journal of Diabetes Mellitus, - http://www.journals.elsevierhealth.com/periodicals/ijdm/inpress DOI: doi:10.1016/j.ijdm 2011.01.007 en_ZA
dc.description Article now published by Lifescience global - http://www.lifescienceglobal.com/home/cart?view=product&id=696 DOI: doi:10.6000/1929-6029.2013.02.03.8 en_ZA
dc.description.abstract Aim: The objective of this study was to investigate the time related profile and efficiency of co-expression of the homeodomain proteins Pdx1, NeuroD, Ngn3, Pax6 and caspase3 with insulin, and to establish the time periods post PDL optimum for islets transplantation. Study design/methods: In this experimental study, immunofluorescent staining procedure was performed on deparaffinized pancreatic duct ligated (PDL) tissues of 78 Sprague–Dawley rats. Quantification of protein coexpression was made using a computerized morphometry. The efficiency of co-expression was arbitrary defined by the value of mean ratio (score without unit) of insulin expression divided by each expression index of the other proteins, occurring within the time interval of 12–24 h post PDL. Statistical tool was used to analyze the efficiency of co-expression of proteins; analysis of variances (one way ANOVA) was used to compare the means of co-expression indexes across the time periods pre- and post PDL. P-values less than 0.05 were considered statistically significant; no post hoc test was done. Results: The curve of insulin expression showed a crossover with that of the co-expression at different time periods pre- and post PDL. The optimal or higher efficiency of co-expression was observed for insulin and Ngn3 co-expression, while a good or medium efficiency was noted for the co-expression of insulin with Pdx1, insulin with NeuroD and insulin with Pax6. Low or weak efficiency was observed for the co-expression of insulin with caspase3. Conclusion: We therefore propose an early islets transplantation using 12–24 h post PDL harvested pancreatic tissues. en_ZA
dc.format 8 p. : ill.
dc.language.iso en_ZA en_ZA
dc.publisher Lifescience global en_ZA
dc.subject Insulin en_ZA
dc.subject Islets transplantation en_ZA
dc.subject Immunofluorescent staining en_ZA
dc.subject Pancreatic duct ligated en_ZA
dc.subject PDL en_ZA
dc.subject Pancreatic tissue en_ZA
dc.title Efficiency of co-expression of transcription factors Pdx1, Ngn3, NeuroD and Pax6 with insulin : a statistical approach en_ZA
dc.type Article en_ZA
dc.description.version Publishers' Version en_ZA
dc.rights.holder Authors retain copyright en_ZA


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