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Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
(BioMed Central, 2013-11)
Background: Starch is one of the most abundant organic polysaccharides available for the production of bio-ethanol as an alternative transport fuel. Cost-effective utilisation of starch requires consolidated bioprocessing ...
Engineering cellulolytic ability into bioprocessing organisms
(Springer-Verlag, 2010-03)
Lignocellulosic biomass is an abundant renewable feedstock for sustainable production of commodities such as biofuels. The main technological barrier that prevents widespread utilization of this resource for production of ...
Enzyme-coupled assay of acetylxylan esterases on monoacetylated 4-nitrophenyl beta-D-xylopyranoside
(Elsevier, 2004-03)
Three different monoacetates of 4-nitrophenyl beta-D-xylopyranoside were tested as substrates for beta -xylosidase and for microbial carbohydrate esterases and a series of non-hemicellulolytic esterases. The acetyl group ...
Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae
(Springer-Verlag, 1999-12)
The xynB gene encoding the Bacillus pumilus beta-xylosidase was expressed separately and jointly with the Trichoderma reesei beta-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the ...
High level secretion of cellobiohydrolases by Saccharomyces cerevisiae
(BioMed Central, 2011-09)
BACKGROUND: The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance. Organisms that hydrolyze ...
Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol
(Elsevier, 2006-09)
To investigate the possible use of Saccharomyces cerevisiae as a candidate for consolidated bioprocessing (CBP) of cellulose to ethanol, four fungal cellobiohydrolase (CBH) encoding genes (Trichoderma reesei cbh1 and cbh2, ...
Role of cultivation media in the development of yeast strains for large scale industrial use
(BioMed Central, 2005-11)
Abstract: The composition of cultivation media in relation to strain development for industrial application is reviewed. Heterologous protein production and pentose utilization by Saccharomyces cerevisiae are used to ...
Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae
(American Society for Microbiology, 1995-04)
The XYN2 gene encoding the main Trichoderma reesei QM 6a endo-beta-1,4-xylanase was amplified by PCR from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was ...
Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger β-xylosidase (xlnD) and the Trichoderma reesei Xylanase II (xyn2) genes
(American Society of Microbiology, 2001-12)
The β-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified ...
Cloning of the Bacillus pumilus beta-xylosidase gene (xynB ) and its expression in Saccharomyces cerevisiae
(Springer-Verlag, 1997-07)
A genomic DNA library of the bacterium Bacillus pumilus PLS was constructed and the beta-xylosidase gene (xynB) was amplified from a 3-kb genomic DNA fragment with the aid of the polymerase chain reaction technique. The ...