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Evaluation of Aspergillus as a host for the production of viral proteins using hepatitis B as a model
(Stellenbosch : Stellenbosch University, 2002-12)
ENGLISH ABSTRACT: Since the advent of recombinant DNA technology in the 1970s, various
microbial hosts have been employed for the efficient high-level heterologous
production of a variety of proteins, ranging from enzymes ...
Cloning of a novel Bacillus pumilus cellobiose-utilising system : functional expression in Escherichia coli
(Stellenbosch : Stellenbosch University, 2002-12)
ENGLISH ABSTRACT: Cellulose, a ~-1,4-linked polymer of glucose, is the most abundant renewable carbon source
on earth. It is well established that efficient degradation of cellulose requires the
synergistic action of ...
Genetic and enzymatic characterisation of wood degrading strains of Phanerochaete species
(Stellenbosch : Stellenbosch University, 2000-03)
ENGLISH ABSTRACT: White rot fungi are of interest in the paper and pulp industry because of their
removal of lignin from wood. In this study over 600 Basidiomycete fungi were
isolated from indigenous forests as well as ...
Engineering of Pichia stipitis for enhanced xylan utilization
(Stellenbosch : Stellenbosch University, 2003-12)
ENGLISH ABSTRACT: Plant biomass, the most abundant renewable resource in nature, consists of matrices of
mainly lignin, cellulose, hemicellulose as well as inorganic components. Xylan, the
major hemicellulose component ...
Heterologous production of family 5 fungal endo-1,4-B-mannanases in Saccharomyces cerevisiae
(Stellenbosch : Stellenbosch University, 2002-12)
ENGLISH ABSTRACT: Mannan polysaccharides occur in the hemicellulose fraction of plant cell walls, either as
structural polymers or as reserve carbohydrates. They are found predominantly in the
seeds of leguminous plants ...
An investigation of the microbial hydrolysis of the lignin carbohydrate complex of grasses
(Stellenbosch : Stellenbosch University, 2004-03)
ENGLISH ABSTRACT: The microbial degradation of the lignin carbohydrate complex of plant material is only
partially understood. Lignin carbohydrate complex was extracted from wheat straw and
subsequently analysed. An ...
Characterization of bacteriocin 423 produced by Lactobacillus pentosus
(Stellenbosch : Stellenbosch University, 2000-12)
ENGLISH ABSTRACT: Worldwide, bacteriocins, particularly those produced by food-related lactic acid bacteria, are
receiving attention due to the possible use of these peptides as natural preservatives in food,
replacing ...
Cloning of a novel esterase gene from Bacillus pumilus and its characterisation in Escherichia coli
(Stellenbosch : Stellenbosch University, 2000-03)
ENGLISH ABSTRACT: Esterases play a variety of roles in nearly every aspect of life ranging from cellular
metabolism, signal transduction to defence mechanisms in plants. One aspect of
esterases that recently is receiving ...
Evaluation and optimisation of fungal enzymes for microbial bioprocessing of rooibos tea
(Stellenbosch : Stellenbosch University, 2005-03)
ENGLISH ABSTRACT: Aspalathus linearis is a leguminous shrub native to the Cedarberg Mountains in the Western
Cape, of which the leaves and stems are used for the preparation of rooibos tea. Over the past
few decades, ...
Evaluation of commercial enzymes for the bioprocessing of Rooibos tea
(Stellenbosch : Stellenbosch University, 2005-04)
ENGLISH ABSTRACT: The Rooibos tea plant (Aspalathus linearis) is indigenous to South Africa and occurs
only in the Western Cape's Cedarberg region. Rooibos tea is produced from the
leaves and fine stems of the plant. ...