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Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up
(Stellenbosch : Stellenbosch University, 2012-12)
ENGLISH ABSTRACT: This study describes:
a) Characterization of traditional methodologies and testing methods used to purify and quantify trypsin and α-chymotrypsin
b) Re-engineering / development of a new method for ...
The optimization of the extraction and purification of horseradish peroxidase from horseradish roots
(Stellenbosch : Stellenbosch University, 2012-12)
ENGLISH ABSTRACT: This study describes:
a) the optimization of the current industrial-scale extraction and purification of Horseradish
peroxidase from horseradish at BBI Enzymes, focussing on:
a. Raw material quality,
b. ...
Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing
(Stellenbosch : Stellenbosch University, 2014-04)
ENGLISH ABSTRACT: This study describes:
the evaluation of the current, and potential assay methods for the quantification of cholesterol, cholesteryl esters and free fatty acids in milk and the application thereof ;
...
The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones
(Stellenbosch : Stellenbosch University, 2015-04)
ENGLISH ABSTRACT: This study describes:
1. Substrate binding assays investigating the effects of methanolic extracts of
unfermented and fermented Rooibos on the binding of natural substrates to ovine
adrenal microsomal ...
The effect of ultraviolet-C treatment on the biochemical composition of beer
(Stellenbosch : Stellenbosch University, 2012-03)
ENGLISH ABSTRACT: This study describes:
· Development of analytical tools to investigate the light struck flavour (LSF) in
beer by Gas chromatography mass spectrometry (GCMS) and by liquid
chromatography mass spectrometry/mass ...
Comparison of two CYP17 isoforms : implications for cortisol production in the South African Merino
(Stellenbosch : Stellenbosch University, 2012-03)
ENGLISH ABSTRACT: This study describes:
• the comparison of the enzymatic activities of the two ovine cytochrome P450 17 -
hydroxylase/17,20-lyase (CYP17) isoforms expressed in non-steroidogenic COS-1 cells.
The Km and ...
The production and purification of functional steroid hormone receptor ligand binding domains towards the development of a biological endocrine disruptor detection system
(Stellenbosch : Stellenbosch University, 2015-04)
ENGLISH ABSTRACT: During the last two and a half decades a large body of research has accumulated indicating the presence of various natural and synthetic chemical compounds within the environment capable of inducing ...
Fabrication and characterization of anti-microbial and biofouling resistant nanofibers with silver nanoparticles and immobilized enzymes for application in water filtration
(Stellenbosch : University of Stellenbosch, 2011-03)
ENGLISH ABSTRACT: Due to a global lack of access to potable water, a problem particularly affecting people in developing countries and the poor, improvement on existing water purification methods are necessary to provide ...
The influence of 3βHSD on adrenal steroidogenesis and the factors which influence its activity
(Stellenbosch : Stellenbosch University, 2012-12)
ENGLISH ABSTRACT: This study describes:
- the characterization and comparison of the enzymatic activity of both Angora and ovine 3βHSD
expressed in non-steroidogenic COS-1 cells. The apparent Km and Vmax values for ...
A biochemical study of tissue type plasminogen activator in bovine milk
(Stellenbosch : Stellenbosch University, 2007-03)
ENGLISH ABSTRACT: This study describes:
1. The isolation and the purification of tissue type plasminogen activator and
urokinase plasminogen activator in bovine milk.
2. The biochemical characterisation of tissue type ...