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Investigation of glucocorticoid and dissociated glucocorticoid activity in hepatoma cell lines with specific reference to regulation of the corticosteroid binding globulin (CBG) proximal promoter'
(Stellenbosch : Stellenbosch University, 2003-12)
ENGLISH ABSTRACT: This study investigated the effect of several hormones on the rat corticosteroid binding
globulin proximal promotor and for the first time showed that modulation occurs at the
promotor level and can ...
An investigation into the stress relieving properties of Sutherlandia frutescens : inhibition of steroidogenic cytochrome P450 enzymes
(Stellenbosch : Stellenbosch University, 2005-12)
ENGLISH ABSTRACT: This study:
I. Investigates the influence of S. frutescens on the binding properties of cytochrome
P450-dependent enzymes in ovine adrenocortical mitochondria and microsomes,
demonstrating that S. ...
Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes
(Stellenbosch : Stellenbosch University, 2005-12)
ENGLISH ABSTRACT: This study describes:
I. The cloning of the CVP 19 gene and construction of the intracellular expression
vector pPIC3.5K-CYP19.
II. The transformation of the yeast, Pichia pastoris with the constructed ...
An investigation into the catalytic activity of porcine cytochrome P450 17α-hydroxylase/17,20-lyase
(Stellenbosch : Stellenbosch University, 2014-04)
ENGLISH ABSTRACT: In this study, the effect of the amino acid residues at positions 40 and 407 on the catalytic activity of
porcine CYP17A1 was investigated. Porcine cofactor CYB5 was cloned from porcine liver tissue ...
The synthesis and characterisation of analogues of the antimicrobial peptide iturin A₂
(Stellenbosch : Stellenbosch University, 1998-12)
ENGLISH ABSTRACT: Iturin A, an antifungal lipopeptide, is produced by Bacillus subtilis. This cyclic peptide consists of seven D- and L-amino acid residues (L-Asn2-D-Tyr3-D-Asn4-L-Gln5-L-Pro6-DAsn7- L-Ser8) and a beta-amino ...
Altered lipid metabolism as a possible mechanism in fumonisin-induced hepatocarcinogenesis in rats and investigations into risk assessment in humans
(Stellenbosch : Stellenbosch University, 2013-12)
ENGLISH ABSTRACT: Exposure to food contaminates such as mycotoxins have been associated with a
variety of animal and human diseases worldwide. In South Africa, maize is the most To further refine risk assessment in the ...
An investigation into the bioactivity of Sutherlandia frutescens (Cancer bush)
(Stellenbosch : University of Stellenbosch, 2009-03)
Sutherlandia frutescens (S. frutescens), sub-species microphylla, is a member of the Fabacea
family and is used as a herbal remedy for the treatment of several ailments which include
influenza, diabetes, cancer, tuberculosis, ...
Identification of two CYP17 alleles in the South African Angora goat
(Stellenbosch : Stellenbosch University, 2003-12)
ENGLISH ABSTRACT: This study describes:
1. The isolation of total RNA and mRNA from Angora goat adrenals.
2. Synthesis and nucleotide sequence alignment of Angora goat CYPI7 cDNA. Two DNA
sequences were produced, ...
The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes
(Stellenbosch : University of Stellenbosch, 2009-03)
This study:
1. Describes the preparation of unfermented and fermented rooibos methanol and aqueous
extracts.
2. Investigates the influence of unfermented and fermented rooibos methanol and aqueous
extracts on the binding ...
Cape baboon Cytochrome P450 11β-hydroxylases : the characterization of two functional enzymes
(Stellenbosch : Stellenbosch University, 2007-03)
ENGLISH ABSTRACT: This study:
1. Describes the localization of CYP11B1 in the Cape baboon adrenal gland using Western
blot analysis. CYP11B1 was localized to the adrenal cortex and medulla.
2. Describes the catalytic ...