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Genotypic and phenotypic analysis of the Env gene from South African HIV-1 subtype B and C isolates

dc.contributor.authorTreurnicht F.K.
dc.contributor.authorSmith T.-L.
dc.contributor.authorEngelbrecht S.
dc.contributor.authorClaassen M.
dc.contributor.authorRobson B.A.
dc.contributor.authorZeier M.
dc.contributor.authorVan Rensburg E.J.
dc.date.accessioned2011-05-15T16:17:03Z
dc.date.available2011-05-15T16:17:03Z
dc.date.issued2002
dc.identifier.citationJournal of Medical Virology
dc.identifier.citation68
dc.identifier.citation2
dc.identifier.issn01466615
dc.identifier.other10.1002/jmv.10199
dc.identifier.urihttp://hdl.handle.net/10019.1/14049
dc.description.abstractThe objective of the study was to assess the genotypic and phenotypic properties of 18 viral strains from human immunodeficiency virus-1 (HIV-1) positive patients and to identify subtype C isolates for vaccine design strategies. All the isolates were non-syncytium-inducing (NSI) in both the primary and MT-2 cell cultures. The amino acid charge of the V3 loop correlated with the NSI phenotype of the strains. The V3 competitive peptide enzyme immunoassay and DNA sequencing of the partial gp120 region gave concordant results on the 15 subtype C strains, whereas the three B genotypes gave a positive to B, a nonreactive to B, and a dual reaction to the B-D peptides, respectively. Sixteen of the isolates used only CCR5 as coreceptor whereas two isolates made use of additional coreceptors including CXCR4. In summary, all our subtype C isolates are NSI phenotypically and almost all of them use CCR5 exclusively as their coreceptor. © 2002 Wiley-Liss, Inc.
dc.subjectchemokine receptor CCR5
dc.subjectchemokine receptor CXCR4
dc.subjectDNA
dc.subjectglycoprotein gp 120
dc.subjectHuman immunodeficiency virus vaccine
dc.subjectamino acid analysis
dc.subjectanimal cell
dc.subjectarticle
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectcorrelation analysis
dc.subjectdrug design
dc.subjectenvelope gene
dc.subjectenzyme immunoassay
dc.subjectgene isolation
dc.subjectgenotype
dc.subjecthuman
dc.subjecthuman cell
dc.subjectHuman immunodeficiency virus 1
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpatient selection
dc.subjectphenotype
dc.subjectSouth Africa
dc.subjectstrain identification
dc.subjectvaccine production
dc.subjectvirus identification
dc.subjectvirus typing
dc.subjectAmino Acid Sequence
dc.subjectCell Line
dc.subjectCytopathogenic Effect, Viral
dc.subjectGenes, env
dc.subjectGenotype
dc.subjectGiant Cells
dc.subjectHIV Envelope Protein gp120
dc.subjectHIV Infections
dc.subjectHIV-1
dc.subjectHumans
dc.subjectMolecular Sequence Data
dc.subjectPeptide Fragments
dc.subjectPhenotype
dc.subjectPhylogeny
dc.subjectReceptors, CCR5
dc.subjectReceptors, CXCR4
dc.subjectSequence Homology, Amino Acid
dc.subjectSouth Africa
dc.subjectHuman immunodeficiency virus
dc.subjectHuman immunodeficiency virus 1
dc.titleGenotypic and phenotypic analysis of the Env gene from South African HIV-1 subtype B and C isolates
dc.typeArticle
dc.description.versionArticle


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