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Novel method for rapid measurement of growth of mycobacteria in detergent-free media

dc.contributor.authorMeyers P.R.
dc.contributor.authorBourn W.R.
dc.contributor.authorSteyn L.M.
dc.contributor.authorVan Helden P.D.
dc.contributor.authorBeyers A.D.
dc.contributor.authorBrown G.D.
dc.date.accessioned2011-05-15T16:16:55Z
dc.date.available2011-05-15T16:16:55Z
dc.date.issued1998
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation36
dc.identifier.citation9
dc.identifier.issn00951137
dc.identifier.urihttp://hdl.handle.net/10019.1/13995
dc.description.abstractWe describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favorably with other methods for monitoring mycobacterial growth but is particularly useful for heavily clumped cultures grown in defined minimal medium.
dc.subjectarticle
dc.subjectbacterial growth
dc.subjectbacterial membrane
dc.subjectbacterium identification
dc.subjectcell aggregation
dc.subjectcentrifugation
dc.subjectmycobacterium bovis
dc.subjectmycobacterium smegmatis
dc.subjectmycobacterium tuberculosis
dc.subjectpriority journal
dc.subjectquantitative diagnosis
dc.subjectreliability
dc.subjectBacterial Proteins
dc.subjectBacteriological Techniques
dc.subjectCulture Media
dc.subjectDetergents
dc.subjectMycobacterium
dc.subjectMycobacterium bovis
dc.subjectMycobacterium tuberculosis
dc.subjectSodium Hydroxide
dc.subjectTime Factors
dc.subjectActinobacteria (class)
dc.subjectBacteria (microorganisms)
dc.subjectCorynebacterineae
dc.subjectMycobacterium bovis
dc.subjectMycobacterium smegmatis
dc.subjectMycobacterium tuberculosis
dc.subjectuncultured actinomycete
dc.titleNovel method for rapid measurement of growth of mycobacteria in detergent-free media
dc.typeArticle
dc.description.versionArticle


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