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The use of short tandem repeat polymorphisms for monitoring chimerism following bone marrow transplantation: A short report

dc.contributor.authorBorrill V.
dc.contributor.authorSchlaphoff T.
dc.contributor.authordu Toit E.
dc.contributor.authorMarx M.
dc.contributor.authorWood L.
dc.contributor.authorJacobs P.
dc.date.accessioned2011-05-15T16:16:30Z
dc.date.available2011-05-15T16:16:30Z
dc.date.issued2008
dc.identifier.citationHematology
dc.identifier.citation13
dc.identifier.citation4
dc.identifier.issn10245332
dc.identifier.other10.1179/102453308X316059
dc.identifier.urihttp://hdl.handle.net/10019.1/13814
dc.description.abstractFollowing immunohaematopoietic stem cell transplantation, it is of importance to determine whether the new blood forming system is of recipient or donor origin and such phenotypic characterisation is called chimerism analysis. This is a dynamic process, which may be complete, mixed or split between compartments and in this way, plays an increasingly important role in predicting outcome for engraftment, rejection or residual disease predicating the need for pre-emptive immunotherapy. Based on recent workshop recommendations, peripheral blood cells have been used in the short tandem repeat (STR) assay to serially characterise the haematologic course and so evaluate the usefulness of this system. Forty-six patients from a single centre were followed serially for periods ranging between 3 and 60 months. The analysis was initially performed using the Applied Biosystems Profiler Plus Kit; currently, the Promega Powerplex 16 system is used. The overlap between the two assays has allowed for continuous comparison. The initial analysis was performed at 14 days post-transplant and repeated monthly. Stored DNA from the patient and donor was used to establish the pre-transplant profile. All post-transplant analyses were performed using peripheral blood. The results obtained were expressed as a percentage of the donor profile. To illustrate the ability of this technology, three representative profiles are described. In the first, stable engraftment is confirmed at 20 months with only donor pattern present. The second is intermediate, and while the patient is clinically disease free, there exists stable mixed chimerism at about 75% of donor cells. The third patient initially engrafted but the reappearance of recipient alleles presaged a haematological relapse; the latter is an indication for salvage with donor lymphocyte infusion and here this assay will be used to show the effectiveness of the intervention. These preliminary results show this to be a useful additional tool in monitoring post-transplant engraftment. As a basis for pro-active therapy, a larger study integrating the results of haematological and cytogenetic markers is planned. © W. S. Maney & Son Ltd 2008.
dc.subjectDNA
dc.subjectadolescent
dc.subjectadult
dc.subjectallele
dc.subjectanalytical equipment
dc.subjectarticle
dc.subjectblood
dc.subjectbone marrow transplantation
dc.subjectcancer relapse
dc.subjectchild
dc.subjectchimera
dc.subjectcytogenetics
dc.subjectdonor
dc.subjectfemale
dc.subjectgenetic polymorphism
dc.subjecthematologic malignancy
dc.subjecthuman
dc.subjectlymphocyte transfusion
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectpriority journal
dc.subjectrecipient
dc.subjectshort tandem repeat
dc.subjectAdolescent
dc.subjectAdult
dc.subjectBone Marrow Transplantation
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectDNA
dc.subjectFemale
dc.subjectHematologic Neoplasms
dc.subjectHumans
dc.subjectMale
dc.subjectMicrosatellite Repeats
dc.subjectMiddle Aged
dc.subjectPolymorphism, Genetic
dc.subjectTissue Donors
dc.subjectTransplantation Chimera
dc.subjectYoung Adult
dc.titleThe use of short tandem repeat polymorphisms for monitoring chimerism following bone marrow transplantation: A short report
dc.typeArticle
dc.description.versionArticle


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