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Time to detection of the growth of Mycobacterium tuberculosis in MGIT 960 for determining the early bactericidal activity of antituberculosis agents

Time to detection of the growth of Mycobacterium tuberculosis in MGIT 960 for determining the early bactericidal activity of antituberculosis agents

dc.contributor.authorDiacon A.H.
dc.contributor.authorMaritz J.S.
dc.contributor.authorVenter A.
dc.contributor.authorVan Helden P.D.
dc.contributor.authorAndries K.
dc.contributor.authorMcNeeley D.F.
dc.contributor.authorDonald P.R.
dc.contributor.authorDiacon A.H.
dc.contributor.authorMaritz J.S.
dc.contributor.authorVenter A.
dc.contributor.authorVan Helden P.D.
dc.contributor.authorAndries K.
dc.contributor.authorMcNeeley D.F.
dc.contributor.authorDonald P.R.
dc.date.accessioned2011-05-15T16:16:19Z
dc.date.accessioned2011-05-15T16:16:19Z
dc.date.available2011-05-15T16:16:19Z
dc.date.available2011-05-15T16:16:19Z
dc.date.issued2010
dc.date.issued2010
dc.identifier.citationEuropean Journal of Clinical Microbiology and Infectious Diseases
dc.identifier.citation29
dc.identifier.citation12
dc.identifier.citationEuropean Journal of Clinical Microbiology and Infectious Diseases
dc.identifier.citation29
dc.identifier.citation12
dc.identifier.issn09349723
dc.identifier.issn09349723
dc.identifier.other10.1007/s10096-010-1043-7
dc.identifier.other10.1007/s10096-010-1043-7
dc.identifier.urihttp://hdl.handle.net/10019.1/13730
dc.identifier.urihttp://hdl.handle.net/10019.1/13730
dc.description.abstractEvaluation of early bactericidal activity (EBA) by the determination of a fall in viable colony-forming units (CFU) of Mycobacterium tuberculosis in sputum is a first step in the clinical study of new antituberculosis agents. The time to detection (TTD) of growth in liquid media is more sensitive and could substitute for CFU counting on solid media. Overnight sputum samples collected during the evaluation of the novel agent TMC207 in comparison to isoniazid and rifampicin were studied. For the determination of CFU, we incubated 10-fold dilutions of homogenized sputum on selective 7H10 agar. The TTD was measured by incubating decontaminated sputum in the BACTEC MGIT 960 system. The fall in bacillary load over 7 days determined by CFU counting closely matched the prolongation of the TTD in the BACTEC MGIT 960 system. The CFU counts correlated significantly with the TTD. While the ranking of agents and different dosages of TMC207 was similar, the highest dose of TMC207 showed markedly better activity when measured by the TTD than CFU counting when compared to the activity of isoniazid. Automated TTD could augment, or, in future, replace, CFU counting to determine sputum bacillary load in EBA clinical trials pending a more formal evaluation of the correlation of the measurements. © 2010 Springer-Verlag.
dc.description.abstractEvaluation of early bactericidal activity (EBA) by the determination of a fall in viable colony-forming units (CFU) of Mycobacterium tuberculosis in sputum is a first step in the clinical study of new antituberculosis agents. The time to detection (TTD) of growth in liquid media is more sensitive and could substitute for CFU counting on solid media. Overnight sputum samples collected during the evaluation of the novel agent TMC207 in comparison to isoniazid and rifampicin were studied. For the determination of CFU, we incubated 10-fold dilutions of homogenized sputum on selective 7H10 agar. The TTD was measured by incubating decontaminated sputum in the BACTEC MGIT 960 system. The fall in bacillary load over 7 days determined by CFU counting closely matched the prolongation of the TTD in the BACTEC MGIT 960 system. The CFU counts correlated significantly with the TTD. While the ranking of agents and different dosages of TMC207 was similar, the highest dose of TMC207 showed markedly better activity when measured by the TTD than CFU counting when compared to the activity of isoniazid. Automated TTD could augment, or, in future, replace, CFU counting to determine sputum bacillary load in EBA clinical trials pending a more formal evaluation of the correlation of the measurements. © 2010 Springer-Verlag.
dc.subjectisoniazid; rifampicin; adult; article; bacterial count; bacterial growth; bactericidal activity; bacterium detection; bacterium examination; clinical article; colony forming unit; controlled study; female; human; liquid culture; male; Mycobacterium tuberculosis; nonhuman; priority journal; sputum culture; time to detection; tuberculosis; Adolescent; Adult; Antitubercular Agents; Colony Count, Microbial; Culture Media; Female; Humans; Isoniazid; Male; Middle Aged; Mycobacterium tuberculosis; Quinolines; Rifampin; Sputum; Time Factors; Treatment Outcome; Tuberculosis, Pulmonary; Young Adult
dc.subjectisoniazid
dc.subjectrifampicin
dc.subjectadult
dc.subjectarticle
dc.subjectbacterial count
dc.subjectbacterial growth
dc.subjectbactericidal activity
dc.subjectbacterium detection
dc.subjectbacterium examination
dc.subjectclinical article
dc.subjectcolony forming unit
dc.subjectcontrolled study
dc.subjectfemale
dc.subjecthuman
dc.subjectliquid culture
dc.subjectmale
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectsputum culture
dc.subjecttime to detection
dc.subjecttuberculosis
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAntitubercular Agents
dc.subjectColony Count, Microbial
dc.subjectCulture Media
dc.subjectFemale
dc.subjectHumans
dc.subjectIsoniazid
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMycobacterium tuberculosis
dc.subjectQuinolines
dc.subjectRifampin
dc.subjectSputum
dc.subjectTime Factors
dc.subjectTreatment Outcome
dc.subjectTuberculosis, Pulmonary
dc.subjectYoung Adult
dc.titleTime to detection of the growth of Mycobacterium tuberculosis in MGIT 960 for determining the early bactericidal activity of antituberculosis agents
dc.titleTime to detection of the growth of Mycobacterium tuberculosis in MGIT 960 for determining the early bactericidal activity of antituberculosis agents
dc.typeArticle
dc.typeArticle
dc.description.versionArticle
dc.description.versionArticle


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