In vitro influence of oxygenation on enzymes of rat adipose tissue.
Date
1992
Authors
Macfarlane C.M.
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Abstract
Pre-incubation of rat adipose tissue from ad lib fed rats in the presence of 1.2 U/ml insulin and 2.8 x 10(-7) M prostaglandin F2 alpha for 16 hours in a gas atmosphere of 5% O2 led to an increase in glucose-6-phosphate dehydrogenase activity. No increase in glycerol-3-phosphate dehydrogenase or hexokinase activities were noted. Actinomycin D (50 micrograms/ml) and dehydroepiandrosterone (120 micrograms/ml) attenuated this increase and the increase in incorporation of [1-14C] glucose into carbon dioxide and tissue seen under these conditions. No increase in glucose-6-phosphate dehydrogenase was seen when pre-incubation was made in a gas atmosphere containing 20% O2. Although dehydroepiandrosterone inhibited both enzyme activity and isotope incorporation in a 20% O2 gas atmosphere, actinomycin D was without effect.
Description
Keywords
dactinomycin, glucose 6 phosphate dehydrogenase, glycerol 3 phosphate dehydrogenase, hexokinase, insulin, oxygen, prasterone, prostaglandin F2 alpha, adipose tissue, animal, article, cell culture, drug effect, enzymology, metabolism, rat, Adipose Tissue, Animal, Cells, Cultured, Dactinomycin, Dinoprost, Glucosephosphate Dehydrogenase, Glycerolphosphate Dehydrogenase, Hexokinase, Insulin, Oxygen, Prasterone, Rats, Support, Non-U.S. Gov't
Citation
Clinical Physiology and Biochemistry
9
2
9
2