A quick, reliable staining technique for human sperm morphology.

Kruger T.F. ; Ackerman S.B. ; Simmons K.F. ; Swanson R.J. ; Brugo S.S. ; Acosta A.A. (1987)


The evaluation of sperm morphology is still an important parameter in the diagnosis of the infertile male. Most techniques used for staining human sperm are very time-consuming. A routine stain used for determining differential count of leucocytes (Diff-Quik stain) was evaluated against the standard Papanicolaou stain. Morphology results from 20 duplicate semen smears using both staining methods were determined separately by 2 technicians using a blind protocol. No significant differences were observed when comparing the two staining methods (paired Student's t-test). The advantages of the Diff-Quik stain are: a) complete staining-to-reading time under 7 min, b) commercially prepared reagents, and c) case of staining procedure.

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/13412
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