A quick, reliable staining technique for human sperm morphology.
Date
1987
Authors
Kruger T.F.
Ackerman S.B.
Simmons K.F.
Swanson R.J.
Brugo S.S.
Acosta A.A.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The evaluation of sperm morphology is still an important parameter in the diagnosis of the infertile male. Most techniques used for staining human sperm are very time-consuming. A routine stain used for determining differential count of leucocytes (Diff-Quik stain) was evaluated against the standard Papanicolaou stain. Morphology results from 20 duplicate semen smears using both staining methods were determined separately by 2 technicians using a blind protocol. No significant differences were observed when comparing the two staining methods (paired Student's t-test). The advantages of the Diff-Quik stain are: a) complete staining-to-reading time under 7 min, b) commercially prepared reagents, and c) case of staining procedure.
Description
Keywords
article, comparative study, human, male, male infertility, methodology, oligospermia, pathology, spermatozoon, staining, Comparative Study, Human, Infertility, Male, Male, Oligospermia, Spermatozoa, Staining and Labeling, Support, Non-U.S. Gov't
Citation
Archives of Andrology
18
3
18
3