Isolation and characterization of the TIM10 homologue from the yeast Pichia sorbitophila a putative component of the mitochondrial protein import system
Date
2000
Authors
Kayingo G.
Potier S.
Hohmann S.
Prior B.A.
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Abstract
The Saccharomyces cerevisiae TIM10 gene encodes one of the few essential mitochondrial proteins that are required for the import of nuclear-encoded precursor proteins from the cytosol and their subsequent sorting into the different mitochondrial compartments. We have isolated and characterized a putative homologue of TIM10 from the halotolerant yeast Pichia sorbitophila. The Pichia TIM10 gene encodes a protein of 90 amino acids with 66% identity to S. cerevisiae Tim10p. It was capable of suppresing the temperature sensitivity of tim10-1 mutant in S. cerevisiae, suggesting that Pichia TIM10 is both a functional and structural homologue of S. cerevisiae TIM10. The putative Pichia TIM10 gene product contains all the four conserved cysteine residues and the two CX3C motifs typical of the Tim family proteins in the mitochondrial intermembrances space. Using anti Tim10p serum, Western blots detected a protein of about 10 kDa, suggesting that the Pichia Tim10p is a mitochondrial protein. The results suggest that mitochondrial import and sorting systems might be also strongly conserved in other fungi. The coding sequence of the P. sorbitophila TIM10 has been deposited in the EMBL Nucleotide Sequence Database under Accession No. AJ243940. Copyright (C) 2000 John Wiley and Sons, Ltd.
Description
Keywords
antiserum, cysteine, fungal protein, gene product, amino acid sequence, article, cytosol, gene isolation, genetic analysis, immunoblotting, mitochondrion, nonhuman, nucleotide sequence, Pichia, Pichia sorbitophila, priority journal, protein transport, Saccharomyces cerevisiae, sequence homology, temperature sensitive mutant, temperature sensitivity, Amino Acid Sequence, Base Sequence, Blotting, Western, Cross Reactions, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Membrane Proteins, Mitochondria, Molecular Sequence Data, Phylogeny, Pichia, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Analysis, DNA
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Yeast
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7
16
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