Differentiation of Leuconostoc species by nicotinamide adenine dinucleotide-dependent D(-)-lactic dehydrogenase profiles
The electrophoretic mobility of the D(-)-nLDH's of Leuconostoc oenos (nine strains), Leuconostoc mesenteroides subsp. mesenteroides (one strain), Leuconostoc mesenteroides subsp. dextranicum (one strain), Leuconostoc lactis (three strains), Leuconostoc paramesenteroides (two strains) and a Leuconostoc sp. were compared using conventional and stacked polyacrylamide gels. A single diffused D(-)-nLDH band was obtained for each strain on a 7% acrylamide gel. However, several well defined D(-)-nLDH bands were obtained for each strain when a polyacrylamide stacked gel was used. Based on D(-)-nLDH profiles, strains of L. oenos were divided into two subgroups. The overall D(-)-nLDH profiles of strains of L. oenos were different from those obtained for the non-acidophilic Leuconostoc spp. Leuconostoc mesenteroides subsp. mesenteroides and L. mesenteroides subsp. dextranicum had similar D(-)-nLDH profiles. The overall D(-)-nLDH profiles of L. lactis corresponded well. Different D(-)-nLDH profiles were obtained for L. paramesenteroides DSM 20201 and the type strain of L. paramesenteroides (DSM 20288(T)). The D(-)-nLDH profile of Leuconostoc sp. ATCC 21436 corresponded well with the profile obtained for the D(-)-nLDH electrophoretic marker of Lactobacillus leichmannii.