Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium
Date
2003
Authors
Donninger H.
Glashoff R.
Haitchi H.-M.
Syce J.A.
Ghildyal R.
Van Rensburg E.
Bardin P.G.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Epithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.
Description
Keywords
alpha chemokine, epithelial derived neutrophil activating factor 78, interleukin 8, messenger RNA, article, asthma, bronchus mucosa, cell activation, cell line, controlled study, disease exacerbation, enzyme linked immunosorbent assay, epithelium cell, gene construct, genetic transcription, human, human cell, human tissue, nonhuman, nucleic acid analysis, priority journal, promoter region, protein function, protein induction, protein secretion, quantitative analysis, reverse transcription polymerase chain reaction, Rhinovirus, virus induction, virus strain, Adult, Asthma, Bronchi, Cell Line, Cells, Cultured, Chemokines, CXC, Epithelium, Female, Gene Expression Regulation, Humans, Interleukin-8, Male, Picornaviridae Infections, Promoter Regions (Genetics), Rhinovirus, RNA, Messenger, Time Factors, Transcription, Genetic, Virus Replication
Citation
Journal of Infectious Diseases
187
11
187
11