Mitochondrial oxidative phosphorylation in myocardial anoxia: effects of albumin
The mechanism whereby albumin exerts its protective action on the oxidative phosphorylation process of mitochondria prepared from anoxic tissue was investigated. Albumin has a beneficial effect on the parameters of mitochondrial function studied, whether present in the mitochondrial isolation medium, mitochondrial incubation medium or perfusate of the isolated anoxic rat heart. The effects of albumin could be removed by perfusing the isolated rat heart with albumin free Ringer. Albumin may act by binding or free fatty acids, which are uncouplers of oxidative phosphorylation. Mitochondria prepared from hearts perfused with palmitate showed a depression of oxidative phosphorylation.