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Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography

dc.contributor.authorde Beer D.
dc.contributor.authorJerz G.
dc.contributor.authorJoubert E.
dc.contributor.authorWray V.
dc.contributor.authorWinterhalter P.
dc.date.accessioned2011-05-15T15:59:34Z
dc.date.available2011-05-15T15:59:34Z
dc.date.issued2009
dc.identifier.citationJournal of Chromatography A
dc.identifier.citation1216
dc.identifier.citation19
dc.identifier.issn219673
dc.identifier.other10.1016/j.chroma.2009.02.056
dc.identifier.urihttp://hdl.handle.net/10019.1/11252
dc.description.abstractIsomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy. © 2009 Elsevier B.V. All rights reserved.
dc.subjectCounter-current chromatography
dc.subjectCyclopia subternata
dc.subjectFabaceae
dc.subjectIsomangiferin
dc.subjectMangiferin
dc.subjectNMR spectroscopy
dc.subjectXanthones
dc.subjectAcetonitrile
dc.subjectBody fluids
dc.subjectBubbles (in fluids)
dc.subjectChromatographic analysis
dc.subjectChromatography
dc.subjectElectrospray ionization
dc.subjectEsters
dc.subjectEthers
dc.subjectHigh performance liquid chromatography
dc.subjectHigh pressure liquid chromatography
dc.subjectLiquids
dc.subjectMethanol
dc.subjectNuclear magnetic resonance
dc.subjectNuclear magnetic resonance spectroscopy
dc.subjectOrganic solvents
dc.subjectPhenols
dc.subjectSolvent extraction
dc.subjectacetic acid ethyl ester
dc.subjectcoumaric acid
dc.subjecteriocitrin
dc.subjecthesperidin
dc.subjectisomangiferin
dc.subjectluteolin
dc.subjectmangiferin
dc.subjectmethanol
dc.subjectphenol derivative
dc.subjectscolymoside
dc.subjectunclassified drug
dc.subjectwater
dc.subjectarticle
dc.subjectcarbon nuclear magnetic resonance
dc.subjectcounter current chromatography
dc.subjectCyclopia subternata
dc.subjectdrug isolation
dc.subjectdrug structure
dc.subjectelectrospray mass spectrometry
dc.subjectextraction
dc.subjectheteronuclear multiple bond correlation
dc.subjecthigh performance liquid chromatography
dc.subjectmedicinal plant
dc.subjectnonhuman
dc.subjectplant leaf
dc.subjectpriority journal
dc.subjectproton nuclear magnetic resonance
dc.subjectChromatography, High Pressure Liquid
dc.subjectCountercurrent Distribution
dc.subjectCyclopia Plant
dc.subjectMethanol
dc.subjectNuclear Magnetic Resonance, Biomolecular
dc.subjectPlant Extracts
dc.subjectXanthones
dc.subjectCyclopia subternata
dc.subjectFabaceae
dc.titleIsolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography
dc.typeArticle
dc.description.versionArticle


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