Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography

SUNScholar Research Repository

Show simple item record

dc.contributor.author de Beer D.
dc.contributor.author Jerz G.
dc.contributor.author Joubert E.
dc.contributor.author Wray V.
dc.contributor.author Winterhalter P.
dc.date.accessioned 2011-05-15T15:59:34Z
dc.date.available 2011-05-15T15:59:34Z
dc.date.issued 2009
dc.identifier.citation Journal of Chromatography A
dc.identifier.citation 1216
dc.identifier.citation 19
dc.identifier.issn 219673
dc.identifier.other 10.1016/j.chroma.2009.02.056
dc.identifier.uri http://hdl.handle.net/10019.1/11252
dc.description.abstract Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy. © 2009 Elsevier B.V. All rights reserved.
dc.subject Counter-current chromatography
dc.subject Cyclopia subternata
dc.subject Fabaceae
dc.subject Isomangiferin
dc.subject Mangiferin
dc.subject NMR spectroscopy
dc.subject Xanthones
dc.subject Acetonitrile
dc.subject Body fluids
dc.subject Bubbles (in fluids)
dc.subject Chromatographic analysis
dc.subject Chromatography
dc.subject Electrospray ionization
dc.subject Esters
dc.subject Ethers
dc.subject High performance liquid chromatography
dc.subject High pressure liquid chromatography
dc.subject Liquids
dc.subject Methanol
dc.subject Nuclear magnetic resonance
dc.subject Nuclear magnetic resonance spectroscopy
dc.subject Organic solvents
dc.subject Phenols
dc.subject Solvent extraction
dc.subject acetic acid ethyl ester
dc.subject coumaric acid
dc.subject eriocitrin
dc.subject hesperidin
dc.subject isomangiferin
dc.subject luteolin
dc.subject mangiferin
dc.subject methanol
dc.subject phenol derivative
dc.subject scolymoside
dc.subject unclassified drug
dc.subject water
dc.subject article
dc.subject carbon nuclear magnetic resonance
dc.subject counter current chromatography
dc.subject Cyclopia subternata
dc.subject drug isolation
dc.subject drug structure
dc.subject electrospray mass spectrometry
dc.subject extraction
dc.subject heteronuclear multiple bond correlation
dc.subject high performance liquid chromatography
dc.subject medicinal plant
dc.subject nonhuman
dc.subject plant leaf
dc.subject priority journal
dc.subject proton nuclear magnetic resonance
dc.subject Chromatography, High Pressure Liquid
dc.subject Countercurrent Distribution
dc.subject Cyclopia Plant
dc.subject Methanol
dc.subject Nuclear Magnetic Resonance, Biomolecular
dc.subject Plant Extracts
dc.subject Xanthones
dc.subject Cyclopia subternata
dc.subject Fabaceae
dc.title Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography
dc.type Article
dc.description.version Article


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record