Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography

Date
2009
Authors
de Beer D.
Jerz G.
Joubert E.
Wray V.
Winterhalter P.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy. © 2009 Elsevier B.V. All rights reserved.
Description
Keywords
Counter-current chromatography, Cyclopia subternata, Fabaceae, Isomangiferin, Mangiferin, NMR spectroscopy, Xanthones, Acetonitrile, Body fluids, Bubbles (in fluids), Chromatographic analysis, Chromatography, Electrospray ionization, Esters, Ethers, High performance liquid chromatography, High pressure liquid chromatography, Liquids, Methanol, Nuclear magnetic resonance, Nuclear magnetic resonance spectroscopy, Organic solvents, Phenols, Solvent extraction, acetic acid ethyl ester, coumaric acid, eriocitrin, hesperidin, isomangiferin, luteolin, mangiferin, methanol, phenol derivative, scolymoside, unclassified drug, water, article, carbon nuclear magnetic resonance, counter current chromatography, Cyclopia subternata, drug isolation, drug structure, electrospray mass spectrometry, extraction, heteronuclear multiple bond correlation, high performance liquid chromatography, medicinal plant, nonhuman, plant leaf, priority journal, proton nuclear magnetic resonance, Chromatography, High Pressure Liquid, Countercurrent Distribution, Cyclopia Plant, Methanol, Nuclear Magnetic Resonance, Biomolecular, Plant Extracts, Xanthones, Cyclopia subternata, Fabaceae
Citation
Journal of Chromatography A
1216
19