Mitochondrial genetic variation reveals phylogeographic structure and cryptic diversity in Trioza erytreae

Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Research (part of Springer Nature)
Abstract
Trioza erytreae is the main vector for ‘Candidatus Liberibacter africanus’, the causative agent of African Citrus Greening disease. The insect is widespread in Africa, and has recently disseminated to Southwestern Europe. This study aimed at generating reference mitogenome sequences for T. erytreae, as a background for future genetic diversity surveys. Complete mitochondrial sequences of three specimens collected in Ethiopia, Uganda and South Africa were recovered using Ion Torrent technology. The mitogenomes of T. erytreae from Uganda and Ethiopia were highly similar, and distinct from that found in South Africa. The phylogeographic structure of T. erytreae was assessed using genetic clustering and pairwise distances, based on a dataset of public COI sequences recorded as T. erytreae. The dataset revealed ten haplotypes with strong phylogeographic structure in Africa and Europe. Three haplotypes found in Kenya on Clausena anisata belonged to pairs separated by distances as high as 11.2%, and were basal to all other sequences. These results indicate that not all sequences identified as T. erytreae belong to the same species, and that some degree of specificity with different plant hosts is likely to exist. This study provides new baseline information on the diversity of T. erytreae, with potential implications for the epidemiology of African Citrus Greening disease.
Description
CITATION: Ajene, I., Pietersen, G. & Van Asch, B. 2020. Mitochondrial genetic variation reveals phylogeographic structure and cryptic diversity in Trioza erytreae. Scientific Reports, 10:8893, doi:10.1038/s41598-020-65880-7.
The original publication is available at https://www.nature.com
Publication of this article was funded by the Stellenbosch University Open Access Fund
Keywords
Mitochondrial genome, Mitochondrial DNA, Mitochondrial genomes, mtDNA, Citrus -- Diseases and pests, Parasites, Insect pests -- Control, Trioza erytreae, Plant diseases, Agricultural pests, Greening disease, Citrus greening disease, Huanglong, Huanglongbin disease, Biological control systems, Molecular diagnosis
Citation
Ajene, I., Pietersen, G. & Van Asch, B. 2020. Mitochondrial genetic variation reveals phylogeographic structure and cryptic diversity in Trioza erytreae. Scientific Reports, 10:8893, doi:10.1038/s41598-020-65880-7