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The development of a medical biomarker test to aid in rapid screening for the presence of infectious diseases

dc.contributor.advisorFourie, P. R.en_ZA
dc.contributor.advisorPerold, W. J.en_ZA
dc.contributor.authorBradford, Dean Johnen_ZA
dc.contributor.otherStellenbosch University. Faculty of Engineering. Dept. of Mechanical and Mechatronic Engineering.en_ZA
dc.date.accessioned2019-02-28T09:59:44Z
dc.date.accessioned2019-04-17T08:29:58Z
dc.date.available2019-02-28T09:59:44Z
dc.date.available2019-04-17T08:29:58Z
dc.date.issued2019-04
dc.identifier.urihttp://hdl.handle.net/10019.1/106126
dc.descriptionThesis (MEng)--Stellenbosch University, 2019.en_ZA
dc.description.abstractENGLISH ABSTRACT: The widespread threat of antimicrobial resistance poses a major problem worldwide. A low-cost rapid biomarker test to aid in the screening of infectious diseases could potentially play a major role in promoting antimicrobial stewardship, thus combating antimicrobial resistance. Here, the prototype development of such a test is reported. C-reactive protein (CRP) and procalcitonin (PCT) were the chosen biomarkers for the test, based on recommendations from literature. Organic electrochemical transistors (OECTs) were chosen for the sensing platform, and were subsequently designed and fabricated using a desktop inkjet printer. The OECT was made sensitive to the target biomarkers by immobilising antibodies, as biorecognition elements, onto the sensor surface. The sensor was then characterised and tested using a National Instruments data acquisition device (DAQ) controlled by a series of LabVIEW programs. The CRP sensors displayed sensitivity to CRP concentrations between 0.3125 ug/mL and 10 ug /mL, whilst the PCT sensors showed no discernible response to the PCT protein. Further work is recommended to enable exact quantification of CRP concentrations, as well as enable the correct detection of PCT proteins.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: Wydverspreide antimikrobiese weerstand is 'n wêreldwye probleem. 'n Goedkoop, vinnige biomerker toets, om te help met die sifting van aansteeklike siektes, kan dus 'n groot rol in die bevordering van antimikrobiese weerstand speel. In hierdie studie word die ontwikkeling van so 'n prototiepe aangemeld. C-reactive protein (CRP) en procalcitonin (PCT) is gekies as biomerkers na aanleiding van aanbevelings vanuit die literatuur studie. Organiese elekrochemiese transistors (OECTs) is gekies vir die sensor platform, en is ontwerp om deur 'n goedkoop huishoudelike drukker vervaardig te word. Die OECT is sensitief tot die teiken biomerkers by ge- ïmmobiliseerde teenligaampies as bio-erkenning elemente, op die sensor oppervlak te gebruik. Die sensor is gekarakteriseer en getoets met 'n National Instruments (NI) data verkrygings toestel (DAQ) en is gekontroleer deur LabVIEW sagteware. Die CRP sensors het sensitiwiteit vir CRP konsentrasies tussen 0.3125 ug/mL en 10 ug/mL gewys, terwyl die PCT sensors geen waarneembare reaksie tot PCT protein gewys het nie. Verdere navorsing om die presiese kwantifisering van CRP konsentrasies aan te dui en die effektiewe opsporing van PCT proteïne vergemaklik word aanbeveel.af_ZA
dc.format.extentxv, 139 pages : illustrationsen_ZA
dc.language.isoen_ZAen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.subjectC-reactive proteinen_ZA
dc.subjectOECTen_ZA
dc.subjectOrganic Electrochemistry Transistorsen_ZA
dc.subjectElectro-organic chemistryen_ZA
dc.subjectNanoscienceen_ZA
dc.subjectNanoscale scienceen_ZA
dc.subjectCommunicable diseasesen_ZA
dc.subjectInfectious diseasesen_ZA
dc.subjectCalcitoninen_ZA
dc.subjectBiosensorsen_ZA
dc.subjectChemical detectorsen_ZA
dc.subjectChemical sensorsen_ZA
dc.subjectUCTD
dc.titleThe development of a medical biomarker test to aid in rapid screening for the presence of infectious diseasesen_ZA
dc.typeThesisen_ZA
dc.rights.holderStellenbosch Universityen_ZA


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