Enzyme kinetics modelling approach to evaluate the impact of high CO2 and super-atmospheric O2 concentrations on respiration rate of pomegranate arils

Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
Super-atmospheric O2 has been shown to affect respiration rate (RR), but no model describing its effect on RR for pomegranate arils has been reported. This study investigated the effects of four different gas compositions (5 kPa O2, 10 kPa CO2 and 85 kPa N2; 10 kPa O2, 5 kPa CO2 and 85 kPa N2; 70 kPa O2, 10 kPa CO2 and 20 kPa N2; and air) on RR of pomegranate arils (cv. Wonderful) stored at 5°C. Michaelis–Menten enzyme kinetics models were used to investigate the effect of CO2 inhibition on O2 consumption rate. Respiratory quotient was used to determine fermentation threshold. The O2 consumption rate increased from 0.87 to 2.81 mL/kg h, with increase in O2 concentration from 5 kPa to 70 kPa. All enzyme kinetics model parameters adequately described the influence of gas concentration on aril RR with correlation coefficient (R2adj = 81–91%).
Description
CITATION: Belay, Z. A., Caleb, O. J. & Opara, U. L. 2017. Enzyme kinetics modelling approach to evaluate the impact of high CO2 and super-atmospheric O2 concentrations on respiration rate of pomegranate arils. CyTA - Journal of Food, 15(4):608-616, doi:10.1080/19476337.2017.1324524.
The original publication is available at https://www.tandfonline.com
Publication of this article was funded by the Stellenbosch University Open Access Fund.
Keywords
Gas bursts, Diffusion
Citation
Belay, Z. A., Caleb, O. J. & Opara, U. L. 2017. Enzyme kinetics modelling approach to evaluate the impact of high CO2 and super-atmospheric O2 concentrations on respiration rate of pomegranate arils. CyTA - Journal of Food, 15(4):608-616, doi:10.1080/19476337.2017.1324524