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Microsatellite-based DNA fingerprinting of selected grapevine cultivars

dc.contributor.authorVan Heerden, C. J.en_ZA
dc.contributor.authorBurger, P.en_ZA
dc.contributor.authorPrins, R.en_ZA
dc.date.accessioned2018-05-15T10:25:12Z
dc.date.available2018-05-15T10:25:12Z
dc.date.issued2018
dc.identifier.citationVan Heerden, C. J., Burger, P. & Prins, R. 2018. Microsatellite-based DNA fingerprinting of selected grapevine cultivars. South African Journal of Enology & Viticulture, 39(1):58-66, doi:10.21548/39-1-2053
dc.identifier.issn2224-7904 (online)
dc.identifier.issn0253-939X (print)
dc.identifier.otherdoi:10.21548/39-1-2053
dc.identifier.urihttp://hdl.handle.net/10019.1/104002
dc.descriptionCITATION: Van Heerden, C. J., Burger, P. & Prins, R. 2018. Microsatellite-based DNA fingerprinting of selected grapevine cultivars. South African Journal of Enology & Viticulture, 39(1):58-66, doi:10.21548/39-1-2053.
dc.descriptionThe original publication is available at http://www.journals.ac.za/index.php/sajev
dc.description.abstractCultivar identification by ampelography is often difficult and is sensitive to environmental conditions, thus it can be problematic to distinguish between closely related cultivars. DNA fingerprinting offers an alternative method that is not influenced by the environment and is relatively easy to perform. However, discriminating between closely related individuals can be problematic if inadequate or insufficient markers are used. Following the estimation of null allele frequencies, an initial set of 35 microsatellite markers was reduced to 20 to generate unique DNA fingerprints for the majority of 111 different grape cultivars and breeding lines. Molecular evidence was utilised to evaluate the accuracy of the reported pedigrees for several cultivars bred in South Africa (SA). The use of markers linked to known downy mildew and powdery mildew resistance loci (Rpv3 and Ren3) provided information regarding the frequency of these resistance loci in the breeding material analysed.en_ZA
dc.description.urihttp://www.journals.ac.za/index.php/sajev/article/view/2053
dc.format.extent9 pages
dc.language.isoen_ZAen_ZA
dc.publisherSouth African Society for Enology and Viticulture
dc.subjectCultivar identificationen_ZA
dc.titleMicrosatellite-based DNA fingerprinting of selected grapevine cultivarsen_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's version
dc.rights.holderSouth African Society for Enology and Viticulture


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