ITEM VIEW

Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization

dc.contributor.authorSun, Jinfengen_ZA
dc.contributor.authorTian, Kangmingen_ZA
dc.contributor.authorWang, Jieen_ZA
dc.contributor.authorDong, Zixingen_ZA
dc.contributor.authorLiu, Xiaoguangen_ZA
dc.contributor.authorPermaul, Kugenthirenen_ZA
dc.contributor.authorSingh, Surenen_ZA
dc.contributor.authorPrior, Bernard A.en_ZA
dc.contributor.authorWang, Zhengxiangen_ZA
dc.date.accessioned2018-05-07T05:36:02Z
dc.date.available2018-05-07T05:36:02Z
dc.date.issued2018-05-02
dc.identifier.citationSun, Jinfeng, et al. 2018. Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization. Microbial Cell Factories, 17:66, doi:10.1186/s12934-018-0915-x
dc.identifier.issn1475-2859 (online)
dc.identifier.otherdoi:10.1186/s12934-018-0915-x
dc.identifier.urihttp://hdl.handle.net/10019.1/103977
dc.descriptionCITATION: Sun, Jinfeng, et al. 2018. Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization. Microbial Cell Factories, 17:66, doi:10.1186/s12934-018-0915-x.
dc.descriptionThe original publication is available at https://microbialcellfactories.biomedcentral.com
dc.description.abstractBackground: Lignocellulosic ethanol could offer a sustainable source to meet the increasing worldwide demand for fuel. However, efficient and simultaneous metabolism of all types of sugars in lignocellulosic hydrolysates by ethanolproducing strains is still a challenge. Results: An engineered strain Escherichia coli B0013-2021HPA with regulated glucose utilization, which could use all monosaccharides in lignocellulosic hydrolysates except glucose for cell growth and glucose for ethanol production, was constructed. In E. coli B0013-2021HPA, pta-ackA, ldhA and pflB were deleted to block the formation of acetate, lactate and formate and additional three mutations at glk, ptsG and manZ generated to block the glucose uptake and catabolism, followed by the replacement of the wild-type frdA locus with the ptsG expression cassette under the control of the temperature-inducible λ pR and pL promoters, and the final introduction of pEtac-PA carrying Zymomonas mobilis pdc and adhB for the ethanol pathway. B0013-2021HPA was able to utilize almost all xylose, galactose and arabinose but not glucose for cell propagation at 34 °C and converted all sugars to ethanol at 42 °C under oxygenlimited fermentation conditions. Conclusions: Engineered E. coli strain with regulated glucose utilization showed efficient metabolism of mixed sugars in lignocellulosic hydrolysates and thus higher productivity of ethanol production.
dc.description.urihttps://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-018-0915-x
dc.format.extent8 pages
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Central
dc.subjectEthanol
dc.titleImproved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilizationen_ZA
dc.typeArticleen_ZA
dc.date.updated2018-05-06T03:38:26Z
dc.description.versionPublisher's version
dc.rights.holderAuthor retains copyright


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

ITEM VIEW