Transposition rates of Mycobacterium tuberculosis IS6110 restriction fragment length polymorphism patterns
Date
2004
Authors
Eilers P.H.C.
Van Soolingen D.
Lan N.T.N.
Warren R.M.
Borgdorff M.W.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
To determine the rate at which IS1160 restriction fragment length polymorphism (RFLP) patterns in Mycobacterium tuberculosis change over time, we applied a smooth nonparametric survival model to several data sets, including data from previous publications on the rate of change. The results strongly suggest a simple parametric model, with an instantaneous change at time zero and essentially a zero rate of change thereafter. Our interpretation of the results is that at the time of collection of the first isolate, more than one strain is present. We speculate that the selection of mutant strains is most likely during rapid growth, revival of the dormant bacteria, and/or adaptation to a new host. The parameter most accurately describing changing RFLP patterns is the proportion of isolates with band changes, rather than the half-life or the rate of change.
Description
Keywords
DNA, Africa, article, bacterium isolate, DNA fingerprinting, DNA transposition, Germany, human, major clinical study, Mycobacterium, Mycobacterium tuberculosis, Netherlands, nonhuman, priority journal, restriction fragment length polymorphism, statistical model, tuberculosis, United States, Viet Nam, DNA Transposable Elements, Mycobacterium tuberculosis, Polymorphism, Restriction Fragment Length, Actinobacteria (class), Bacteria (microorganisms), Mycobacterium, Mycobacterium tuberculosis, uncultured actinomycete
Citation
Journal of Clinical Microbiology
42
6
42
6