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Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity

dc.contributor.authorWarren R.
dc.contributor.authorDe Kock M.
dc.contributor.authorEngelke E.
dc.contributor.authorMyburgh R.
dc.contributor.authorVan Pittius N.G.
dc.contributor.authorVictor T.
dc.contributor.authorVan Helden P.
dc.date.accessioned2011-05-15T15:56:56Z
dc.date.available2011-05-15T15:56:56Z
dc.date.issued2006
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation44
dc.identifier.citation1
dc.identifier.issn951137
dc.identifier.other10.1128/JCM.44.1.254-256.2006
dc.identifier.urihttp://hdl.handle.net/10019.1/10120
dc.description.abstractConcern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
dc.subjectbacterial DNA
dc.subjectarticle
dc.subjectbactericidal activity
dc.subjectbacterium culture
dc.subjectDNA extraction
dc.subjectDNA fingerprinting
dc.subjectDNA isolation
dc.subjectheat
dc.subjectinfection control
dc.subjectmolecular epidemiology
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectBacteriological Techniques
dc.subjectDNA, Bacterial
dc.subjectHeat
dc.subjectHumans
dc.subjectLaboratory Infection
dc.subjectMycobacterium Infections
dc.subjectMycobacterium tuberculosis
dc.subjectSafety Management
dc.subjectBacteria (microorganisms)
dc.subjectCorynebacterineae
dc.subjectMycobacterium tuberculosis
dc.titleSafe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity
dc.typeArticle
dc.description.versionArticle


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