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Improvement in mycobacterial yield and reduced time to detection in pediatric samples by use of a nutrient broth growth supplement

dc.contributor.authorBrittle W.
dc.contributor.authorMarais B.J.
dc.contributor.authorHesseling A.C.
dc.contributor.authorSchaaf H.S.
dc.contributor.authorKidd M.
dc.contributor.authorWasserman E.
dc.contributor.authorBotha T.
dc.date.accessioned2011-05-15T15:56:54Z
dc.date.available2011-05-15T15:56:54Z
dc.date.issued2009
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation47
dc.identifier.citation5
dc.identifier.issn951137
dc.identifier.other10.1128/JCM.02320-08
dc.identifier.urihttp://hdl.handle.net/10019.1/10105
dc.description.abstractThere is an urgent need to improve the methods used for the bacteriological diagnosis of childhood mycobacterial disease. This study compared the mycobacterial yields and the times to detection (in days) of mycobacteria in pediatric clinical specimens by using Mycobacterial Growth Indicator Tubes (MGITs) and solid Löwenstein-Jensen (LJ) slants with and without a nutrient broth supplement. A total of 801 specimens from 493 patients were processed: 82.8% were gastric aspirate specimens, 15.6% were sputum specimens, and 1.6% were fine-needle-aspiration biopsy specimens. The mycobacterial yield obtained with MGITs (with and without nutrient broth) was 11.0%, and that obtained with LJ slants was 1.6% (P < 0.001). Of the 88 positive cultures, 62 were detected in MGITs and 73 were detected in MGITs supplemented with nutrient broth (P = 0.11). The mean time to detection in MGITs (without nutrient broth) was 18.5 days, whereas it was 12.4 days in MGITs with nutrient broth (P < 0.001). Supplementation of standard MGITs improved the mycobacterial yield and significantly reduced the time to detection of mycobacteria in pediatric samples. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
dc.subjectadolescent
dc.subjectarticle
dc.subjectaspiration biopsy
dc.subjectbacterium detection
dc.subjectbroth dilution
dc.subjectchild
dc.subjectchildhood disease
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectfemale
dc.subjectgastric suction
dc.subjecthuman
dc.subjectinfant
dc.subjectlaboratory diagnosis
dc.subjectlowenstein jensen slant
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmycobacterial growth indicator tube
dc.subjectMycobacterium
dc.subjectpreschool child
dc.subjectpriority journal
dc.subjectschool child
dc.subjectsputum culture
dc.subjecttuberculosis
dc.subjectAdolescent
dc.subjectBacteriological Techniques
dc.subjectBiopsy, Fine-Needle
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectCulture Media
dc.subjectFemale
dc.subjectGastric Juice
dc.subjectHumans
dc.subjectInfant
dc.subjectInfant, Newborn
dc.subjectMale
dc.subjectMycobacterium
dc.subjectSputum
dc.subjectTime Factors
dc.subjectTuberculosis
dc.subjectCorynebacterineae
dc.titleImprovement in mycobacterial yield and reduced time to detection in pediatric samples by use of a nutrient broth growth supplement
dc.typeArticle
dc.description.versionArticle


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