Characterization and heterologous expression of a class IIa bacteriocin, plantaricin 423 from Lactobacillus plantarum 423, in Saccharomyces cerevisiae

Date
2003
Authors
Van Reenen C.A.
Chikindas M.L.
Van Zyl W.H.
Dicks L.M.T.
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Abstract
Lactobacillus plantarum 423 produces a small heat-stable antimicrobial protein designated plantaricin 423. This protein is bactericidal for many Gram-positive foodborne pathogens and spoilage bacteria, including Listeria spp., Staphylococcus spp., Pediococcus spp., Lactobacillus spp., etc. The DNA sequence of the plantaricin 423-encoding region on plasmid pPLA4 revealed a four open reading frame (ORF) operon structure similar to pediocin PA-1/AcH from Pediococcus acidilactici and coagulin from Bacillus coagulans I4. The first ORF, plaA, encodes a 56-amino acid prepeptide consisting of a 37-amino acid mature molecule, with a 19-amino acid N-terminal leader peptide. The second ORF, plaB, encodes a putative immunity protein with protein sequence similarities to several bacteriocin immunity proteins. The plaC and plaD genes are virtually identical to pedC and pedD of the pediocin PA-1 operon, as well as coaC and coaD of the coagulin operon. Plantaricin 423 was cloned on a shuttle vector under the control of a yeast promoter and heterologously produced in Saccharomyces cerevisiae. © 2002 Elsevier Science B.V. All rights reserved.
Description
Keywords
amino acid, bacterial protein, bacteriocin, coagulin, food preservative, pediocin, peptide, plantaricin, unclassified drug, amino acid sequence, amino terminal sequence, article, Bacillus coagulans, bacterial gene, bacterial strain, bacterium, controlled study, DNA sequence, Lactobacillus plantarum, molecular cloning, molecule, nonhuman, open reading frame, operon, pedicoccus acidilacti, plasmid, protein analysis, protein expression, Saccharomyces cerevisiae, sequence homology, shuttle vector, Amino Acid Sequence, Bacteriocins, Base Sequence, DNA, Bacterial, Lactobacillus, Molecular Sequence Data, Open Reading Frames, Plasmids, Saccharomyces cerevisiae, Spectrometry, Mass, Electrospray Ionization, Bacillus coagulans, Lactobacillus, Lactobacillus plantarum, Listeria, Pediococcus, Pediococcus acidilactici, Posibacteria, Saccharomyces, Saccharomyces cerevisiae, Staphylococcus, unidentified shuttle vector
Citation
International Journal of Food Microbiology
81
1