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Phenotypic analysis of peripheral B cell populations during mycobacterium tuberculosis infection and disease

dc.contributor.authorDu Plessis, Willem J.en_ZA
dc.contributor.authorKeyser, Alanaen_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.date.accessioned2017-01-24T11:30:07Z
dc.date.available2017-01-24T11:30:07Z
dc.date.issued2016-07-29
dc.identifier.citationDu Plessis, W. J., et al. 2016. Phenotypic analysis of peripheral B cell populations during mycobacterium tuberculosis infection and disease. Journal of Inflammation, 13:23, doi:10.1186/s12950-016-0133-4en_ZA
dc.identifier.issn1476-9255 (Online)
dc.identifier.issn1476-9255 (Print)
dc.identifier.otherdoi:10.1186/s12950-016-0133-4
dc.identifier.urihttp://hdl.handle.net/10019.1/100514
dc.descriptionCITATION: Du Plessis, W. J., et al. 2016. Phenotypic analysis of peripheral B cell populations during mycobacterium tuberculosis infection and disease. Journal of Inflammation, 13:23, doi:10.1186/s12950-016-0133-4.en_ZA
dc.descriptionThe original publication is available at http://health-policy-systems.biomedcentral.comen_ZA
dc.description.abstractBackground: Mycobacterium tuberculosis (Mtb) remains an unresolved threat resulting in great annual loss of life. The role of B cells during the protective immunity to Mtb is still unclear. B cells have been described as effector cells in addition to their role as antibody producing cells during disease. Here we aim to identify and characterize the frequency of peripheral B-cell subpopulations during active Tuberculosis and over treatment response. Analysis were done for both class switched (CS) and non-class switched (NCS) phenotypes. Methods: We recruited participants with active untreated pulmonary Tuberculosis, other lung diseases and healthy community controls. All groups were followed up for one week from recruitment and the TB cases till the end of treatment (month 6). Results: Peripheral blood samples were collected, stained with monoclonal antibodies to CD19+ cells, Immunoglobulin (Ig) M, plasma cells (CD 138+), marker of memory (CD27+), immune activation (CD23+) and acquired on a flow cytometer. Circulating Marginal zone B cells (CD19+IgM+CD23−CD27+) and memory phenotypes are able to distinguish between TB diagnosis and end of treatment. The frequency of mature B cells from TB cases are lower than that of other-lung diseases at diagnosis. A subpopulation of activated memory B cells (CD19+IgM+CD23+CD27+) cells are present at the end of TB treatment. Conclusions: This study identified distinctive B cell subpopulations present during active TB disease and other lung disease conditions. These cell populations warrants further examination in larger studies as it may be informative as cell markers or as effectors/regulators in TB disease or TB treatment response.en_ZA
dc.description.urihttp://health-policy-systems.biomedcentral.com/articles/10.1186/s12961-016-0089-0
dc.format.extent8 pages : illustrations (some colour)en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.subjectB cells -- Analysisen_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subjectBiochemical markersen_ZA
dc.subjectTuberculosis -- Immune responseen_ZA
dc.subjectPlasma cellsen_ZA
dc.titlePhenotypic analysis of peripheral B cell populations during mycobacterium tuberculosis infection and diseaseen_ZA
dc.typeArticleen_ZA
dc.date.updated2016-12-09T12:10:28Z
dc.description.versionPublisher's versionen_ZA
dc.rights.holderAuthor retains copyrighten_ZA


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