Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA of Lactobacillus plantarum 423, monitored with real-time PCR

Date
2007
Authors
Ramiah K.
van Reenen C.A.
Dicks L.M.T.
Journal Title
Journal ISSN
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Abstract
Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA by Lactobacillus plantarum 423, grown in the presence of bile, pancreatin and at low pH, was studied by real-time PCR. Mub, MapA and EF-Tu were up-regulated in the presence of mucus, proportional to increasing concentrations. Expression of MapA was up-regulated in the presence of 3.0 g/l bile and 3.0 g/l pancreatin at pH 6.5. Similar results were recorded in the presence of 10.0 g/l bile and 10.0 g/l pancreatin at pH 6.5. Expression of Mub was down-regulated in the presence of bile and pancreatin, whilst the expression of EF-Tu and plaA remained unchanged. Expression of Mub and MapA remained unchanged at pH 4.0, whilst expression of EF-Tu and plaA were up-regulated. Expression of MapA was down-regulated in the presence of 1.0 g/l l-cysteine HCl, suggesting that the gene is regulated by transcription attenuation that involves cysteine. © 2007 Elsevier B.V. All rights reserved.
Description
Keywords
bacteriocin, cysteine, hydrochloric acid, pancreatin, article, bile, controlled study, down regulation, gene control, gene expression, genetic transcription, Lactobacillus plantarum, mucus, nonhuman, nucleotide sequence, pH, real time polymerase chain reaction, upregulation, Bacterial Adhesion, Bacterial Proteins, Bile, Gene Expression Regulation, Bacterial, Humans, Hydrogen-Ion Concentration, Lactobacillus plantarum, Mucus, Pancreatin, Polymerase Chain Reaction, Probiotics, Lactobacillus plantarum
Citation
International Journal of Food Microbiology
116
3