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Analysis for a limited number of gene codons can predict drug resistance of Mycobacterium tuberculosis in a high-incidence community

Van Rie A.; Warren R.; Mshanga I.; Jordaan A.M.; Van Der Spuy G.D.; Richardson M.; Simpson J.; Gie R.P.; Enarson D.A.; Beyers N.; Van Helden P.D.; Victor T.C. (2001)

Correct and rapid diagnosis is essential in the management of multidrug-resistant tuberculosis (MDR-TB). In this population-based study of 61 patients with drug-resistant tuberculosis, we evaluated the frequency of mutations ...

Calculation of the stability of the IS6110 banding pattern in patients with persistent Mycobacterium tuberculosis disease

Warren R.M.; Van der Spuy G.D.; Richardson M.; Beyers N.; Borgdorff M.W.; Behr M.A.; Van Helden P.D. (2002)

The interpretation of molecular epidemiologic data of Mycobacterium tuberculosis infection is dependent on the understanding of the stability and evolutionary characteristics of the DNA fingerprinting marker used to classify ...

Evolution of the IS6110-based restriction fragment length polymorphism pattern during the transmission of Mycobacterium tuberculosis

Warren R.M.; Van der Spuy G.D.; Richardson M.; Beyers N.; Booysen C.; Behr M.A.; Van Helden P.D. (2002)

Interpretation of the molecular epidemiological data of Mycobacterium tuberculosis is dependent on the validity of the assumptions that have been made. It is assumed that the IS6110 banding pattern is sufficiently stable ...

Polymerase chain reaction in the diagnosis of urinary tract tuberculosis

Van Vollenhoven P.; Heyns C.F.; De Beer P.M.; Whitaker P.; Van Helden P.D.; Victor T. (1996)

The polymerase chain reaction (PCR) is a technique that can be used to amplify a specific DNA genomic sequence, whereby the presence of an extremely small number of bacteria can be detected. The high sensitivity of PCR is ...

Beijing and Haarlem genotypes are overrepresented among children with drug-resistant tuberculosis in the Western Cape province of South Africa

Marais B.J.; Victor T.C.; Hesseling A.C.; Barnard M.; Jordaan A.; Brittle W.; Reuter H.; Beyers N.; Van Helden P.D.; Warren R.M.; Schaaf H.S. (2006)

Drug resistance among children with culture-confirmed tuberculosis (TB) provides an accurate measure of transmitted drug resistance within the community. We describe the genotype diversity in children with culture-confirmed ...

Genome-wide analysis of the structure of the South African Coloured Population in the Western Cape

De Wit E.; Delport W.; Rugamika C.E.; Meintjes A.; Moller M.; Van Helden P.D.; Seoighe C.; Hoal E.G. (2010)

Admixed populations present unique opportunities to discover the genetic factors underlying many multifactorial diseases. The geographical position and complex history of South Africa has led to the establishment of the ...

Molecular bacterial load assay, a culture-free biomarker for rapid and accurate quantification of sputum Mycobacterium tuberculosis bacillary load during treatment

Honeyborne I.; McHugh T.D.; Phillips P.P.J.; Bannoo S.; Bateson A.; Carroll N.; Perrin F.M.; Ronacher K.; Wright L.; Van Helden P.D.; Walzl G.; Gillespie S.H. (2011)

Isoniazid pharmacokinetics in children treated for respiratory tuberculosis

Schaaf H.S.; Parkin D.P.; Seifart H.I.; Werely C.J.; Hesseling P.B.; Van Helden P.D.; Maritz J.S.; Donald P.R. (2005)

Aims: To define the pharmacokinetics of isoniazid (INH) in children with tuberculosis in relation to the N-acetyltransferase 2 (NAT2) genotype. Methods: The first order elimination rate constant (k) and area under the ...

Purification of sputum samples through sucrose improves detection of mycobacterium tuberculosis by polymerase chain reaction

Victor T.; Du Toit R.; Van Helden P.D. (1992)

A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent ...

Association between tuberculosis and a polymorphic NFκB binding site in the interferon γ gene

Rossouw M.; Nel H.J.; Cooke G.S.; Van Helden P.D.; Hoal E.G. (2003)

Interferon γ is believed to be crucial for host defence against many infections. To test the hypothesis that a polymorphism in the gene for interferon γ (IFNG) is associated with susceptibility to tuberculosis, we did two ...