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Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium

Gorgens J.F. Van Zyl W.H. Knoetze J.H. Hahn-Hagerdal B. (2005)

Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase ...

Bioprospecting for beta-glucosidases and beta-xylosidases from non-Saccharomyces yeast

Omardien, Soraya (Stellenbosch : Stellenbosch University, 2013-03)

ENGLISH ABSTRACT: The argument of whether to use food for biofuel (bioethanol) production prompted the search for an alternative non-food biomass, such as lignocellulose, as feedstock for bioethanol production. However, ...

Carnitine biosynthesis in Neurospora crassa: Identification of a cDNA coding for ε-N-trimethyllysine hydroxylase and its functional expression in Saccharomyces cerevisiae

Swiegers J.H. Vaz F.M. Pretorius I.S. Wanders R.J.A Bauer F.F. (2002)

The biosynthesis of L-carnitine in eukaryotic organisms was first elucidated in the ascomycete Neurospora crassa. The first step of the pathway is catalysed by ε-N-trimethyllysine hydroxylase (TMLH), which converts ...

Characterization and heterologous expression of a class IIa bacteriocin, plantaricin 423 from Lactobacillus plantarum 423, in Saccharomyces cerevisiae

Van Reenen C.A. Chikindas M.L. Van Zyl W.H. Dicks L.M.T. (2003)

Lactobacillus plantarum 423 produces a small heat-stable antimicrobial protein designated plantaricin 423. This protein is bactericidal for many Gram-positive foodborne pathogens and spoilage bacteria, including Listeria ...

Cloning and characterization of a second α-amylase gene (LKA2) from Lipomyces kononenkoae IGC4052B and its expression in Saccharomyces cerevisiae

Eksteen J.M. Steyn A.J.C. van Rensburg P. Cordero Otero R.R. Pretorius I.S. (2003)

Lipomyces kononenkoae secretes a battery of highly effective amylases (i.e. α-amylase, glucoamylase, isoamylase and cyclomaltodextrin glucanotransferase activities) and is therefore considered as one of the most efficient ...

Comparative characterization of endo-polygalacturonase (Pgu1) from Saccharomyces cerevisiae and Saccharomyces paradoxus under winemaking conditions

Eschstruth A. Divol B. (2011)

Wine strains of Saccharomyces cerevisiae have no to weak natural pectinase activity, despite their genetic ability to secrete an endo-polygalacturonase. The addition of external pectinase of fungal origin has therefore ...

Differences among AGT1-encoded α-glucoside transporters and their ability to transport maltotriose in Saccharomyces yeasts

Smit A. Cordero Otero R.R. Pretorius I.S. (2007)

Improved fermentation of starch and its dextrin products would benefit the brewing and whiskey industries. Most strains of Saccharomyces ferment glucose and maltose and partially ferment maltotriose, but are unable to ...

Differential malic acid degradation by selected strains of Saccharomyces during alcoholic fermentation

Redzepovic S. Orlic S. Majdak A. Kozina B. Volschenk H. Viljoen-Bloom M. (2003)

To produce a high-quality wine, it is important to obtain a fine balance between the various chemical constituents, especially between the sugar and acid content. The latter is more difficult to achieve in wines that have ...

Discrepancy in glucose and fructose utilisation during fermentation by Saccharomyces cerevisiae wine yeast strains

Berthels N.J. Cordero Otero R.R. Bauer F.F. Thevelein J.M. Pretorius I.S. (2004)

While unfermented grape must contains approximately equal amounts of the two hexoses glucose and fructose, wine producers worldwide often have to contend with high residual fructose levels (>2 gl-1) that may account for ...

Enhancing volatile phenol concentrations in wine by expressing various phenolic acid decarboxylase genes in Saccharomyces cerevisiae

Smit A. Cordero Otero R.R. Lambrechts M.G. Pretorius I.S. Van Rensburg P. (2003)

Phenolic acids, which are generally esterified with tartaric acid, are natural constituents of grape must and wine and can be released as free acids (principally p-coumaric, caffeic, and ferulic acids) by certain cinnamoyl ...

Evaluation of polygalacturonase activity in Saccharomyces cerevisiae wine strains

Fernandez-Gonzalez M. Ubeda J.F. Vasudevan T.G. Otero R.R.C. Briones A.I. (2004)

A total of 61 S. cerevisiae strains, 60 of them isolated from wine ecosystems, were evaluated for the presence of the gene encoding endopolygalacturonase (PGU1) and for polygalacturonase (PG) activity. Nine strains lack ...

Expression of the Aspergillus niger glucose oxidase gene in Saccharomyces cerevisiae and its potential applications in wine production

Malherbe D.F. Du Toit M. Cordero Otero R.R. Van Rensburg P. Pretorius I.S. (2003)

There is a growing consumer demand for wines containing lower levels of alcohol and chemical preservatives. The objectives of this study were to express the Aspergillus niger gene encoding a glucose oxidase (GOX; ...

Generation of the improved recombinant xylose-utilizing Saccharomyces cerevisiae TMB 3400 by random mutagenesis and physiological comparison with Pichia stipitis CBS 6054

Wahlbom C.F. Van Zyl W.H. Jonsson L.J. Hahn-Hagerdal B. Cordero Otero R.R. (2003)

The recombinant xylose-utilizing Saccharomyces cerevisiae TMB 3399 was constructed by chromosomal integration of the genes encoding D-xylose reductase (XR), xylitol dehydrogenase (XDH), and xylulokinase (XK). S. cerevisiae ...

Implications of FPS1 deletion and membrane ergosterol content for glycerol efflux from Saccharomyces cerevisiae

Toh T.-H. Kayingo G. Van Der Merwe M.J. Kilian S.G. Hallsworth J.E. Hohmann S. Prior B.A. (2001)

The deletion of the gene encoding the glycerol facilitator Fps1p was associated with an altered plasma membrane lipid composition in Saccharomyces cerevisiae. The S. cerevisiae fps1Δ strain respectively contained 18 and ...

Localization of yeast glucoamylase genes by PFGE and OFAGE

Pretorius I.S. Marmur J. (1988)

[No abstract available]

Malo-ethanolic fermentation in Saccharomyces and Schizosaccharomyces

Volschenk H. van Vuuren H.J.J. Viljoen-Bloom M. (2003)

Yeast species are divided into the K(+) or K(-) groups, based on their ability or inability to metabolise tricarboxylic acid (TCA) cycle intermediates as sole carbon or energy source. The K(-) group of yeasts includes ...

Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol

Becker J.V.W. Armstrong G.O. Van Der Merwe M.J. Lambrechts M.G. Vivier M.A. Pretorius I.S. (2003)

The stilbene resveratrol is a stress metabolite produced by Vitis vinifera grapevines during fungal infection, wounding or UV radiation. Resveratrol is synthesised particularly in the skins of grape berries and only trace ...

Molecular analysis of a Saccharomyces cerevisiae mutant with improved ability to utilize xylose shows enhanced expression of proteins involved in transport, initial xylose metabolism, and the pentose phosphate pathway

Wahlbom C.F. Cordero Otero R.R. Van Zyl W.H. Hahn-Hagerdal B. Jonsson L.J. (2003)

Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by ...

Optimization of a rapid method for studying the cellular location of β-glucosidase activity in wine yeasts

Arevalo Villena M. Ubeda Iranzo J.F. Cordero Otero R.R. Briones Perez A.I. (2005)

Aims: To improve a method for determining β-glucosidase activity and to apply it in yeasts isolated from wine ecosystems from 'La Mancha' region and to know its cellular location. Methods and Results: A total of 82 wine ...

Overexpression, secretion and antifungal activity of the Saccharomyces cerevisiae chitinase

Carstens M. Vivier M.A. Van Rensburg P. Pretorius I.S. (2003)

The excessive use of pesticides to prevent the outbreak of plant diseases holds various disadvantages for the cost-effectiveness of agricultural production practices, the quality of the end products, the environment and ...

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